Department of Genetics, College of Science, Kazerun Branch, Islamic Azad University, Kazerun, Iran.
Department of Medical Laboratory Sciences, Faculty of Medicine, Kazerun Branch, Islamic Azad University, Kazerun, Iran.
BMC Pregnancy Childbirth. 2023 Dec 6;23(1):839. doi: 10.1186/s12884-023-06143-x.
Preeclampsia is the main cause of preterm parturition and maternal-fetal complications. T helper 1 and T helper 2 cytokines balance is a requirement in normal pregnancy and aberrant in this immunologic balance, play an important role in the pathology of preeclampsia. In previous studies single nucleotide polymorphisms have been associated with the alteration of serum cytokine levels.
This study was aimed to discover association between interleukin-13 (rs20541, and rs56035208) and interleukin-19 (rs1028181 (T/C) and rs2243191(T/C)) polymorphisms with susceptibility to preeclampsia.
In this case-control study 300 women with and without preeclampsia (n = 150/each) who referred to Zeynabieh Hospital- Shiraz, Iran, from February 2021 to April 2022 were enrolled. For genotyping the interleukin-13 and interleukin-19 polymorphisms, the Allele-specific polymerase chain reaction and direct sequencing method was carried out.
Our statistical results revealed no significant differences in allele and genotype frequencies for interleukin-13 polymorphisms compared to controls. We found that the interleukin-13 polymorphisms are significantly associated with vulnerability to edema at rs20541 position and maternal drinking at rs56035208 position. But it was interesting to note that the differences of both the allele and genotype frequencies of interleukin-19 polymorphisms and their contribution to the risk of preeclampsia susceptibility were significant.
No risk of preeclampsia was found in all comparisons for interleukin-13 polymorphisms. However, the interleukin-19 polymorphisms were found to confer the risk of preeclampsia in our population.
子痫前期是早产和母婴并发症的主要原因。辅助性 T 细胞 1 和辅助性 T 细胞 2 细胞因子平衡是正常妊娠的要求，而这种免疫平衡的异常在子痫前期的病理中起着重要作用。在之前的研究中，单核苷酸多态性与血清细胞因子水平的改变有关。
本研究旨在发现白细胞介素-13(rs20541 和 rs56035208)和白细胞介素-19(rs1028181(T/C)和 rs2243191(T/C))多态性与子痫前期易感性的关系。
在这项病例对照研究中，我们招募了 300 名患有和不患有子痫前期的女性(每组 150 名)，她们于 2021 年 2 月至 2022 年 4 月期间在伊朗设拉子的 Zeynabieh 医院就诊。为了进行白细胞介素-13 和白细胞介素-19 多态性的基因分型，我们使用了等位基因特异性聚合酶链反应和直接测序法。
我们的统计结果显示，白细胞介素-13 多态性的等位基因和基因型频率与对照组相比没有显著差异。我们发现，白细胞介素-13 多态性与 rs20541 位置的水肿易感性和 rs56035208 位置的母亲饮酒有关。但有趣的是，白细胞介素-19 多态性的等位基因和基因型频率及其对子痫前期易感性的风险贡献都有显著差异。
在所有比较中，白细胞介素-13 多态性与子痫前期的风险无关。然而，白细胞介素-19 多态性在我们的人群中与子痫前期的风险有关。
