Department of Pharmaceutical Chemistry and Biomaterials, Faculty of Pharmacy, Medical University of Warsaw, ul. Banacha 1, 02-097 Warsaw, Poland.
Department of Toxicology and Bromatology, Faculty of Pharmacy, Medical University of Warsaw, ul. Banacha 1, 02-097 Warsaw, Poland.
Int J Mol Sci. 2023 Nov 25;24(23):16751. doi: 10.3390/ijms242316751.
This study presents a comprehensive evaluation of novel composite biomaterials designed for dental applications, aiming to potentially address the prevalent challenge of dental and periodontal tissue loss. The composites consisted of biomimetic hydroxyapatite (mHA) enriched with Mg, CO, and Zn ions, type I collagen, alginate, and, additionally, chitosan and sericin. The granules were loaded with ibuprofen sodium salt. The investigation encompassed a morphology characterization, a porosity analysis, a chemical structure assessment, and an examination of the swelling behavior, drug release kinetics (ibuprofen), and release profiles of zinc and magnesium ions. The granules exhibited irregular surfaces with an enhanced homogeneity in the chitosan-coated granules and well-developed mesoporous structures. The FT-IR spectra confirmed the presence of ibuprofen sodium, despite overlapping bands for the polymers. The granules demonstrated a high water-absorption capacity, with delayed swelling observed in the chitosan-coated granules. Ibuprofen displayed burst-release profiles, especially in the G1 and G3 samples. In the case of the chitosan-coated granules (G2 and G4), lower amounts of ibuprofen were released. In turn, there was a significant difference in the released amount of magnesium and zinc ions from the granules, which was most likely caused by their different location in the hydroxyapatite crystals. The cytotoxicity assays confirmed the non-cytotoxic behavior of the biomaterial. These findings suggest the potential applicability of these biomaterials in dental scenarios, emphasizing their multifunctional and biocompatible nature.
本研究全面评估了设计用于牙科应用的新型复合生物材料，旨在潜在解决牙齿和牙周组织损失的普遍问题。复合材料由仿生羟基磷灰石（mHA）组成，富含 Mg、CO 和 Zn 离子、I 型胶原、海藻酸盐，此外还含有壳聚糖和丝胶。颗粒中负载了布洛芬钠盐。研究包括形貌表征、孔隙率分析、化学结构评估以及溶胀行为、药物释放动力学（布洛芬）和锌离子和镁离子释放曲线的研究。颗粒表面呈不规则状，壳聚糖涂层颗粒的均匀性提高，具有发达的中孔结构。FT-IR 光谱证实了布洛芬钠的存在，尽管聚合物的谱带存在重叠。颗粒具有高吸水性，壳聚糖涂层颗粒的溶胀延迟。布洛芬呈现出突释释放曲线，特别是在 G1 和 G3 样品中。在壳聚糖涂层颗粒（G2 和 G4）中，释放的布洛芬量较少。相反，颗粒中镁和锌离子的释放量存在显著差异，这很可能是由于它们在羟基磷灰石晶体中的位置不同所致。细胞毒性试验证实了生物材料的非细胞毒性行为。这些发现表明这些生物材料在牙科领域具有潜在的应用前景，强调了它们的多功能和生物相容性。
