Hampton W A, Townsend M C, Haybron D M, Shirmer W J, Fry D E
J Surg Res. 1987 Jan;42(1):33-8. doi: 10.1016/0022-4804(87)90061-8.
To assess the effects of sepsis on effective hepatic blood flow (EHBF) and hepatic tissue bioenergy status 250-350 g rats underwent either sham laparotomy or cecal ligation and perforation (CLP). At 5-, 10-, and 20-hr intervals cardiac output (CO), EHBF, and tissue adenine nucleotide levels were measured. CLP rats showed a hyperdynamic response to sepsis at 20 hr, with CO increased by 25% over sham rats. At all time intervals studied EHBF was decreased and at 20 hr it was maximally decreased by 35%. Hepatic energy charge (HEC) was calculated from tissue adenine nucleotide measurements. HEC was not statistically different in sham and CLP rats at 5 hr and was 11 and 9% reduced from sham levels in CLP rats at 10 and 20 hr, respectively. Thus, EHBF is decreased very early in sepsis; before any changes in hepatic bioenergy status are noted. In this hyperdynamic model of sepsis EHBF is decreased early and decreases progressively with time which may contribute to the significant decrease in HEC that is demonstrated.
为评估脓毒症对有效肝血流量（EHBF）和肝组织生物能状态的影响，对250 - 350克的大鼠进行了假剖腹手术或盲肠结扎穿孔术（CLP）。在术后5小时、10小时和20小时的时间间隔测量心输出量（CO）、EHBF和组织腺嘌呤核苷酸水平。CLP大鼠在20小时时对脓毒症表现出高动力反应，其CO比假手术大鼠增加了25%。在所有研究的时间间隔内，EHBF均降低，在20小时时最大降幅为35%。肝能荷（HEC）由组织腺嘌呤核苷酸测量值计算得出。在5小时时，假手术大鼠和CLP大鼠的HEC无统计学差异，在10小时和20小时时，CLP大鼠的HEC分别比假手术大鼠水平降低了11%和9%。因此，在脓毒症早期EHBF就降低了；在肝生物能状态出现任何变化之前。在这个脓毒症高动力模型中，EHBF早期降低且随时间逐渐下降，这可能导致了所显示的HEC的显著降低。
