Chaudry I H, Wichterman K A, Baue A E
Surgery. 1979 Feb;85(2):205-11.
Tissue adenine nucleotides were measured in rats to determine if there is depletion of energy stores associated with sepsis. Peritonitis was produced by cecal ligation and cecal puncture. At 16 to 24 hours after ligation, rats which were lethargic but still normotensive (late sepsis) and showed clinical and laboratory confirmation of peritonitis-sepsis were stunned by a blow on the head, and small pieces of tissue were removed and frozen. Adenine nucleotides were measured enzymatically. In late sepsis adenosine triphosphate (ATP) levels in liver and kidney decreased significantly; however, no significant decreases were observed in the diaphragm or gastrocnemius muscle. Hydrogen polarograph measurements of hepatic blood flow indicated that flow was decreased markedly at this stage of peritonitis. A second group of rats was prepared in the same manner, except they were studied 10 hours after ligation (early sepsis). Most rats at this stage of sepsis appeared to be only mildly ill; however, blood cultures obtained from six rats so prepared all were positive. These rats did not show any decrease in either hepatic blood flow or tissue adenine nucleotides. Thus the changes in adenine nucleotides observed in late sepsis (lpw-flow septic rats) are similar to those seen during early hemorrhagic shock and suggest inadequate perfusion associated with peritonitis as the cause.
对大鼠的组织腺嘌呤核苷酸进行了测量，以确定是否存在与败血症相关的能量储备耗竭。通过盲肠结扎和盲肠穿刺制造腹膜炎。在结扎后16至24小时，对那些嗜睡但仍血压正常（晚期败血症）且临床和实验室检查证实为腹膜炎 - 败血症的大鼠，通过头部打击使其昏迷，然后取出小块组织并冷冻。用酶法测量腺嘌呤核苷酸。在晚期败血症中，肝脏和肾脏中的三磷酸腺苷（ATP）水平显著下降；然而，在膈肌或腓肠肌中未观察到显著下降。肝血流的氢极谱测量表明，在腹膜炎的这个阶段血流明显减少。以同样的方式制备了第二组大鼠，只是在结扎后10小时（早期败血症）对它们进行研究。在败血症的这个阶段，大多数大鼠似乎只是轻度患病；然而，从如此制备的6只大鼠获得的血培养均为阳性。这些大鼠在肝血流或组织腺嘌呤核苷酸方面均未显示任何下降。因此，在晚期败血症（低血流败血症大鼠）中观察到的腺嘌呤核苷酸变化与早期失血性休克时所见相似，提示与腹膜炎相关的灌注不足是原因。
