Yang Da, An Jia, Qiu Wu, Gao Yuhan, Zhang Jiajing, Pan Wencai, Zhao Peng, Liu Yufei
Key Laboratory of Optoelectronic Technology & Systems (Chongqing University), Ministry of Education, Chongqing, 400044, China.
School of Microelectronics, Southern University of Science and Technology, Shenzhen, 518055, China.
Mikrochim Acta. 2023 Dec 13;191(1):22. doi: 10.1007/s00604-023-06101-6.
A paper-based electrochemical dual-function biosensor capable of determining pH and TCO was synthesized for the first time using an iridium oxide pH electrode and an all-solid-state ion electrode (ASIE). In the study, to obtain highly reliable results, the biosensor was equipped with a real-time pH correction function before TCO measurements. Compared to traditional liquid-filling carbon dioxide detection sensors, the utilization of ferrocene endows our novel sensor with abundant positive sites, and thus greatly improves its performance. Conversely, the introduction of MXene with conductivity close to that of metals reduces electrode resistance, which is beneficial for accelerating the electrochemical reaction of the sensor and reducing LOD. After optimization, the detection range of TCO is 0.095 nM-0.66 M, with a detection limit of as low as 0.023 nM. In addition, the sensor was used in real serum sample-spiked recovery experiments and comparison experiments with existing clinical blood gas analyzers, which confirmed the effectiveness of its clinical application. This study provides a method for the rational design of paper-based electrochemical biosensors and a new approach for the clinical detection of blood carbon dioxide.
首次使用氧化铱pH电极和全固态离子电极（ASIE）合成了一种能够测定pH值和总二氧化碳（TCO）的纸质电化学双功能生物传感器。在该研究中，为了获得高度可靠的结果，该生物传感器在进行TCO测量之前配备了实时pH校正功能。与传统的液体填充式二氧化碳检测传感器相比，二茂铁的使用赋予了我们的新型传感器丰富的正位点，从而极大地提高了其性能。相反，引入导电性接近金属的MXene降低了电极电阻，这有利于加速传感器的电化学反应并降低检测限（LOD）。经过优化，TCO的检测范围为0.095 nM - 0.66 M，检测限低至0.023 nM。此外，该传感器用于实际血清样品加标回收实验以及与现有临床血气分析仪的比较实验，证实了其临床应用的有效性。本研究为纸质电化学生物传感器的合理设计提供了一种方法，并为血液二氧化碳的临床检测提供了一种新途径。
