School of Natural Science, University of Galway, Galway H91 TK33, Ireland.
Anses, Ploufragan-Plouzané-Niort Laboratory, Mycoplasmology-Bacteriology and Antimicrobial Resistance Unit, 22440 Ploufragan, France.
Dis Aquat Organ. 2023 Dec 14;156:115-121. doi: 10.3354/dao03766.
This work generates the data needed to set epidemiological cut-off values for disc-diffusion zone measurements of Vibrio cholerae. The susceptibility of 147 European isolates of non-O1/non-O139 V. cholerae to 19 antibiotics was established using a standardised disc diffusion method which specified incubation of Mueller Hinton agar plates at 35°C. Epidemiological cut-off values were calculated by analysis of the zone size data with the statistically based normalised resistance interpretation method. Cut-off values for 17 agents were calculated by analysis of the aggregated data from all 4 laboratories participating in this study. The cut-off values calculated were ≥18 mm for amoxicillin/clavulanate, ≥18 mm for amikacin, ≥19 mm for ampicillin, ≥27 mm for cefepime, ≥31 mm for cefotaxime, ≥24 mm for ceftazidime, ≥24 mm for chloramphenicol, ≥31 mm for ciprofloxacin, ≥16 mm for erythromycin, ≥ 27 mm for florfenicol, ≥16 mm for gentamicin, ≥23 mm for imipenem, ≥25 mm for meropenem, ≥29 mm for nalidixic acid, ≥28 mm for norfloxacin, ≥13 mm for streptomycin and ≥23 mm for tetracycline. For the other 2 agents the data from 1 laboratory was excluded from the censored aggregation because the data from that laboratory was considered excessively imprecise. The cut-off values for these 2 agents calculated for the aggregation of the data from 3 laboratories were ≥23 mm for trimethoprim and ≥24 mm for trimethoprim/sulfamethoxazole. These zone size data will be submitted to the Clinical Laboratory Standards Institute (CLSI) and European Committee for Antimicrobial Susceptibility Testing (EUCAST) for their consideration in setting international consensus epidemiological cut-off values for non O1/non-O139 V. cholerae.
这项工作生成了设置霍乱弧菌碟扩散带测量的流行病学截断值所需的数据。使用标准化的碟扩散法测定了 147 株欧洲非 O1/非 O139 霍乱弧菌对 19 种抗生素的敏感性，该方法规定在 35°C 下孵育 Mueller Hinton 琼脂平板。通过分析所有 4 个参与本研究的实验室的汇总数据，采用基于统计学的归一化耐药解释方法对带大小数据进行分析，计算出流行病学截断值。通过分析本研究中所有 4 个实验室汇总数据，计算出 17 种药物的截断值。计算出的截断值为：阿莫西林/克拉维酸≥18mm、阿米卡星≥18mm、氨苄西林≥19mm、头孢吡肟≥27mm、头孢噻肟≥31mm、头孢他啶≥24mm、氯霉素≥24mm、环丙沙星≥31mm、红霉素≥16mm、氟苯尼考≥27mm、庆大霉素≥16mm、亚胺培南≥23mm、美罗培南≥25mm、萘啶酸≥29mm、诺氟沙星≥28mm、链霉素≥13mm、四环素≥23mm。对于另外两种药物，由于来自一个实验室的数据被认为过于不准确，因此从包含的数据中排除了该实验室的数据。对来自 3 个实验室的数据进行汇总分析后，计算出这两种药物的截断值分别为：甲氧苄啶≥23mm 和复方磺胺甲噁唑≥24mm。这些带大小数据将提交给临床实验室标准化协会（CLSI）和抗菌药物敏感性测试欧洲委员会（EUCAST），供其考虑为非 O1/非 O139 霍乱弧菌设定国际共识流行病学截断值。
