Cheng Sheena Yi-Hsin, Huang Shih-Yuan, Cheng Shih-Ping
Taipei European School, Taipei, Taiwan.
Department of Medical Research, MacKay Memorial Hospital, Taipei, Taiwan.
Front Toxicol. 2023 Nov 30;5:1294760. doi: 10.3389/ftox.2023.1294760. eCollection 2023.
Several ecological studies suggest that ambient air pollution is associated with the occurrence of thyroid cancer. In this study, we used certified diesel particulate matter as a proxy for fine particulate matter. Human thyroid cancer cell lines 8505C and TPC-1 were incubated with different concentrations of NIST1650b for 5 days and subjected to functional assays. We found that NIST1650b treatment did not affect short-term cell growth but reduced colony formation at high concentrations. Notably, NIST1650b-treated cells showed altered morphology toward cluster coalescence following treatment. Wound healing assays revealed that leading-edge cells formed protruding tips while maintaining cell-cell adhesion, and a significantly higher ratio of wound closure following treatment at 10 μg/mL was seen in both cell lines. A weak stimulatory effect on transwell cell migration was observed in 8505C cells. Taken together, our results suggest that fine particulate matter induced a coherent phenotype accompanied by augmented collective cell migration in thyroid cancer cells.
多项生态学研究表明，环境空气污染与甲状腺癌的发生有关。在本研究中，我们使用经认证的柴油颗粒物作为细颗粒物的替代物。将人甲状腺癌细胞系8505C和TPC-1与不同浓度的NIST1650b孵育5天，并进行功能测定。我们发现，NIST1650b处理不影响细胞短期生长，但在高浓度时会减少集落形成。值得注意的是，经NIST1650b处理的细胞在处理后显示出向细胞簇融合改变的形态。伤口愈合试验表明，前沿细胞形成突出的尖端，同时保持细胞间粘附，并且在两种细胞系中，10μg/mL处理后伤口闭合率显著更高。在8505C细胞中观察到对Transwell细胞迁移有微弱的刺激作用。综上所述，我们的结果表明，细颗粒物在甲状腺癌细胞中诱导了一种连贯的表型，并伴随着集体细胞迁移增加。
