National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
Plant Biotechnol J. 2024 May;22(5):1352-1371. doi: 10.1111/pbi.14271. Epub 2023 Dec 15.
Lipids and lipid metabolites have essential roles in plant-pathogen interactions. GDSL-type lipases are involved in lipid metabolism modulating lipid homeostasis. Some plant GDSLs modulate lipid metabolism altering hormone signal transduction to regulate host-defence immunity. Here, we functionally characterized a rice lipase, OsGELP77, promoting both immunity and yield. OsGELP77 expression was induced by pathogen infection and jasmonic acid (JA) treatment. Overexpression of OsGELP77 enhanced rice resistance to both bacterial and fungal pathogens, while loss-of-function of osgelp77 showed susceptibility. OsGELP77 localizes to endoplasmic reticulum and is a functional lipase hydrolysing universal lipid substrates. Lipidomics analyses demonstrate that OsGELP77 is crucial for lipid metabolism and lipid-derived JA homeostasis. Genetic analyses confirm that OsGELP77-modulated resistance depends on JA signal transduction. Moreover, population genetic analyses indicate that OsGELP77 expression level is positively correlated with rice resistance against pathogens. Three haplotypes were classified based on nucleotide polymorphisms in the OsGELP77 promoter where OsGELP77 is an elite haplotype. Three OsGELP77 haplotypes are differentially distributed in wild and cultivated rice, while OsGELP77 has been broadly pyramided for hybrid rice development. Furthermore, quantitative trait locus (QTL) mapping and resistance evaluation of the constructed near-isogenic line validated OsGELP77, a QTL for broad-spectrum disease resistance. In addition, OsGELP77-modulated lipid metabolism promotes JA accumulation facilitating grain yield. Notably, the hub defence regulator OsWRKY45 acts upstream of OsGELP77 by initiating the JA-dependent signalling to trigger immunity. Together, OsGELP77, a QTL contributing to immunity and yield, is a candidate for breeding broad-spectrum resistant and high-yielding rice.
脂质和脂质代谢物在植物-病原体相互作用中具有重要作用。GDSL 型脂肪酶参与调节脂质稳态的脂质代谢。一些植物 GDSLs 通过改变激素信号转导来调节脂质代谢，从而调节宿主防御免疫。在这里，我们功能表征了一种水稻脂肪酶 OsGELP77，它既能促进免疫又能提高产量。OsGELP77 的表达受病原体感染和茉莉酸 (JA) 处理诱导。过表达 OsGELP77 增强了水稻对细菌和真菌病原体的抗性，而 osgelp77 的功能丧失则表现出易感性。OsGELP77 定位于内质网，是一种水解通用脂质底物的功能性脂肪酶。脂质组学分析表明，OsGELP77 对脂质代谢和脂质衍生的 JA 稳态至关重要。遗传分析证实，OsGELP77 调节的抗性取决于 JA 信号转导。此外，群体遗传学分析表明，OsGELP77 表达水平与水稻对病原体的抗性呈正相关。根据 OsGELP77 启动子中的核苷酸多态性，将 OsGELP77 分为三个单倍型，其中 OsGELP77 是一个优良单倍型。三个 OsGELP77 单倍型在野生和栽培稻中分布不同，而 OsGELP77 已广泛用于杂交稻的发展。此外，构建的近等基因系的数量性状位点 (QTL) 定位和抗性评价验证了 OsGELP77 是广谱抗病的 QTL。此外，OsGELP77 调节的脂质代谢促进 JA 积累，从而提高产量。值得注意的是，枢纽防御调节剂 OsWRKY45 通过启动 JA 依赖的信号转导来触发免疫，从而在上游作用于 OsGELP77。综上所述，OsGELP77 是一个既能提高免疫又能提高产量的 QTL，是培育广谱抗性和高产水稻的候选基因。
