Tian Bingren, Qiao Xia, Guo Songlin, Li Aiqin, Xu Yanan, Cao Jia, Zhang Xu, Ma Duan
Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China.
Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China.
Int J Biol Macromol. 2024 Feb;257(Pt 2):128719. doi: 10.1016/j.ijbiomac.2023.128719. Epub 2023 Dec 13.
The development of nanoparticles loaded with natural active ingredients is one of the hot trends in the pharmaceutical industry. Herein, chitosan was selected as the base material, and sodium tripolyphosphate was chosen as the cross-linking agent. Chitosan nanoparticles loaded with β-acids from hops were prepared by the ionic cross-linking method. The results of Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) indicated that chitosan nanoparticles successfully encapsulated β-acids. The loading capacity of chitosan nanoparticles with β-acids was 2.00 %-18.26 %, and the encapsulation efficiency was 0.58 %-55.94 %. Scanning electron microscopy (SEM), transmission electron microscope (TEM), particle size, and zeta potential results displayed that the nanoparticles revealed a sphere-like distribution with a particle size range of 241-261 nm, and the potential exhibited positive potential (+14.47-+16.27 mV). The chitosan nanoparticles could slowly release β-acids from different simulated release media. Notably, the β-acids-loaded nanoparticles significantly inhibited Staphylococcus aureus ATCC25923 (S. aureus) and Escherichia coli ATCC25922 (E. coli). Besides, β-acids-loaded chitosan nanoparticles were cytotoxic to colorectal cancer cells (HT-29 and HCT-116). Therefore, applying chitosan nanoparticles can further expand the application of β-acids in biomedical fields.
负载天然活性成分的纳米颗粒的研发是制药行业的热门趋势之一。在此，选择壳聚糖作为基材，并选用三聚磷酸钠作为交联剂。采用离子交联法制备了负载啤酒花β-酸的壳聚糖纳米颗粒。傅里叶变换红外光谱（FTIR）和X射线衍射（XRD）结果表明壳聚糖纳米颗粒成功包封了β-酸。壳聚糖纳米颗粒对β-酸的负载量为2.00%-18.26%，包封率为0.58%-55.94%。扫描电子显微镜（SEM）、透射电子显微镜（TEM）、粒径和zeta电位结果显示，纳米颗粒呈球状分布，粒径范围为241-261nm，电位呈正电位（+14.47-+16.27mV）。壳聚糖纳米颗粒可从不同模拟释放介质中缓慢释放β-酸。值得注意的是，负载β-酸的纳米颗粒显著抑制金黄色葡萄球菌ATCC25923（金黄色葡萄球菌）和大肠杆菌ATCC25922（大肠杆菌）。此外，负载β-酸的壳聚糖纳米颗粒对结肠癌细胞（HT-29和HCT-116）具有细胞毒性。因此，应用壳聚糖纳米颗粒可进一步拓展β-酸在生物医学领域的应用。
