Stefan-van Staden Raluca-Ioana, Stefan Andrada-Ioana, Georgescu-State Ramona, Gifu Ioana Catalina
Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Street, 060021, Bucharest, Romania.
National Research and Development Institute for Chemistry and Petrochemistry ICECHIM, 202 Splaiul Independentei Street, 060021, Bucharest, Romania.
Mikrochim Acta. 2025 Sep 5;192(10):637. doi: 10.1007/s00604-025-07514-1.
Molecular recognition and determination of vascular cell adhesion molecule-1 (VCAM-1), interleukin-6 (IL-6), and natriuretic peptide C-type (NPPC) are essential for the early prognosis and diagnosis of cardiovascular diseases, especially in young obese populations. Highly sensitive and selective devices characterized by low Limits of quantification are required for their determination in whole blood. Therefore, a 3D stochastic sensor was developed by immobilizing a chitosan hydrogel onto a carbon paste electrode (used as the support matrix for the hydrogel), which was subsequently modified with gold nanoparticles, multi-walled carbon nanotubes, and β-cyclodextrin (β-CD/AuNPs@MWCNT/CS/CPE). The sensor was designed, characterized, and validated using whole blood samples and compared with commercial enzyme-linked immunoassay (ELISA) kits for the molecular recognition and determination of IL-6, VCAM-1, and NPPC. The sensor exhibited low Limits of quantification: 4.10 × 10 g mL for IL-6, 5.00 × 10 g mL for VCAM-1, and 1.00 × 10 g mL for NPPC, with wide Linear concentration ranges of 4.10 × 10-2.00 × 10 g mL (IL-6), 5.00 × 10-5.00 × 10 g mL (VCAM-1), and 1.00 × 10-1.00 × 10 g mL (NPPC). The recoveries for each biomarker were higher than 99.85%, with relative standard deviations below 1.00%. The sensor can be used as a screening tool for whole blood, enabling rapid molecular recognition and determination of IL-6, VCAM-1, and NPPC.
血管细胞黏附分子-1（VCAM-1）、白细胞介素-6（IL-6）和C型利钠肽（NPPC）的分子识别与测定对于心血管疾病的早期预后和诊断至关重要，尤其是在年轻肥胖人群中。在全血中测定它们需要具有低定量限的高灵敏度和选择性设备。因此，通过将壳聚糖水凝胶固定在碳糊电极（用作水凝胶的支撑基质）上开发了一种3D随机传感器，随后用金纳米颗粒、多壁碳纳米管和β-环糊精对其进行修饰（β-CD/AuNPs@MWCNT/CS/CPE）。该传感器使用全血样本进行设计、表征和验证，并与用于IL-6、VCAM-1和NPPC分子识别与测定的商业酶联免疫吸附测定（ELISA）试剂盒进行比较。该传感器表现出低定量限：IL-6为4.10×10 g/mL，VCAM-1为5.00×10 g/mL，NPPC为1.00×10 g/mL，线性浓度范围宽，分别为4.10×10 - 2.00×10 g/mL（IL-6）、5.00×10 - 5.00×10 g/mL（VCAM-1）和1.00×10 - 1.00×10 g/mL（NPPC）。每个生物标志物的回收率均高于99.85%，相对标准偏差低于1.00%。该传感器可作为全血的筛查工具，能够快速进行IL-6、VCAM-1和NPPC的分子识别与测定。
