Parasitology and Animal Diseases Department, Veterinary Research Institute, National Research Centre, 33 El Buhouth St. (former El-Tahrir St.), Dokki, Giza, P.O. 12622, Egypt.
Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh, 33516, Egypt.
Parasitol Res. 2023 Dec 16;123(1):55. doi: 10.1007/s00436-023-08063-3.
Ninety-seven (64.67%) out of 150 domestic goats (Capra hiricus) carcasses were found to be infected by Sarcocystis moulei, Sarcocystis capracanis, and Sarcocystis hircicanis sarcocysts. Sarcocystis moulei macrosarcocysts were detected in the cardiac, esophageal, skeletal, lingual, and diaphragmatic muscles of seven goats (4.67%) out of the 150 examined animals, whereas the microscopic Sarcocystis species were found in (90/150 = 60%). Two morphotypes of S. moulei were observed. Morphotype (I) macrosarcocysts were large-sized oval, ovoid, spherical, and measured 2-7 mm in length x 2-6 mm in width. Sarcocystis moulei morphotype (II) macrosarcocysts were spindle-shaped, spheroid, sometimes elongated, and measured 1.8-6 x 0.5-2 mm. By TEM, all S. moulei morphotypes were ultrastructurally the same and had a sarcocyst wall that was characterized by highly branched or cauliflower-like villar protrusions (VP) with dumbbell-like structures. The VP interior was packed with well-developed microtubules in longitudinal and cross arrangements. Sarcocystis moulei cyst wall was 3-6 μm thick. Sarcocystis capracanis microsarcocysts detected herein had a cyst wall that ranged from 4-8 μm in thickness. The VP was upright finger-like or cylindrical. The PVM had electron-dense corrugations in the region of the VP. Few amounts of microfilaments were detected inside the cores of VP. Sarcocystis hircicanis had a thinner cyst wall (~1-3 μm) with hairy long VP that ranged from 1 to 7.5 μm in length. Microtubules were missing inside the cores of the VP. The three caprine Sarcocystis species were molecularly characterized on the level of the 18S rRNA, 28S rRNA, and Cox1 genes.
在 150 只本地山羊（Capra hiricus）的胴体中，有 97 只（64.67%）被检测到感染了莫氏弓形虫、卡氏弓形虫和赫氏弓形虫的包囊。在检查的 150 只动物中，有 7 只（4.67%）的心脏、食管、骨骼肌、舌和膈肌中检测到莫氏弓形虫的大型包囊，而显微镜下的弓形虫物种则在 90/150=60%的动物中被发现。观察到两种莫氏弓形虫形态。形态（I）大型包囊呈长椭圆形、卵圆形、球形，长 2-7 毫米，宽 2-6 毫米。莫氏弓形虫形态（II）大型包囊呈梭形、球形，有时呈长形，长 1.8-6 毫米，宽 0.5-2 毫米。通过 TEM，所有莫氏弓形虫形态在超微结构上都是相同的，具有特征性的分支状或菜花状绒毛状突起（VP）的囊壁，具有哑铃状结构。VP 内部排列着纵横交错的发育良好的微管。莫氏弓形虫囊壁厚 3-6 微米。本文检测到的卡氏弓形虫微包囊的囊壁厚度为 4-8 微米。VP 直立呈指状或圆柱形。PVM 在 VP 区域有电子致密的波纹。在 VP 核心内部检测到少量的微丝。赫氏弓形虫的囊壁较薄（~1-3 微米），VP 有长长的毛发，长 1 至 7.5 微米。VP 核心内部没有微管。这三种山羊弓形虫种在 18S rRNA、28S rRNA 和 Cox1 基因水平上进行了分子特征分析。
