从黄曲霉毒素降解菌中克隆脂肪酶基因及其重组酶的降解特性。

Mining Lactonase Gene from Aflatoxin B-Degrading Strain and Degrading Properties of the Recombinant Enzyme.

机构信息

College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, People's Republic of China.

Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Zhengzhou, Henan 450001, People's Republic of China.

出版信息

J Agric Food Chem. 2023 Dec 27;71(51):20762-20771. doi: 10.1021/acs.jafc.3c05725. Epub 2023 Dec 16.

DOI:10.1021/acs.jafc.3c05725

PMID:38103014

Abstract

Mycotoxins are toxic secondary metabolites mainly produced by filamentous fungal species that commonly contaminate food and feed. Aflatoxin B (AFB) is extremely toxic and seriously threatens the health of humans and animals. In this work, the HNGD-A6 was obtained and showed a 94.66% removal ability of AFB by employing extracellular enzymes as the degrading active substance. The degradation products were P1 (AFD, CHO) and P2 (CHNO), and their toxicity was greatly reduced compared to that of AFB. The AttM gene was mined by BlastP comparison and successfully expressed in BL21. AttM could degrade 86.78% of AFB at pH 8.5 and 80 °C, as well as 81.32% of ochratoxin A and 67.82% of zearalenone. The ability of AttM to degrade a wide range of toxins and its resistance to high temperatures offer the possibility of its use in food or feed applications.

摘要

真菌毒素是由丝状真菌物种产生的有毒次生代谢物，通常会污染食物和饲料。黄曲霉毒素 B（AFB）毒性极强，严重威胁人类和动物的健康。在这项工作中，使用细胞外酶作为降解活性物质，获得了 HNGD-A6，其对 AFB 的去除能力达到了 94.66%。降解产物为 P1（AFD，CHO）和 P2（CHNO），与 AFB 相比，其毒性大大降低。通过 BlastP 比较挖掘了 AttM 基因，并在 BL21 中成功表达。AttM 可以在 pH8.5 和 80°C 下降解 86.78%的 AFB、81.32%的赭曲霉毒素 A 和 67.82%的玉米赤霉烯酮。AttM 降解多种毒素的能力及其对高温的耐受性为其在食品或饲料中的应用提供了可能性。