Department of Physics-Biophysics, Faculty of Science, New Valley University, New Valley 72511, Egypt.
Department of Physics, Faculty of Applied Science, Umm AL-Qura University, Makkah 24382, Saudi Arabia.
Biochim Biophys Acta Gen Subj. 2024 Mar;1868(3):130543. doi: 10.1016/j.bbagen.2023.130543. Epub 2023 Dec 14.
Novel biocompatible and effective hyperthermia (HT) treatment materials for breast cancer therapeutic have recently attracting researchers, because of their effective ablation of cancer cells and negligible damage to healthy cells. Magnetoliposome (MLs) have numerous possibilities for utilize in cancer treatment, including smart drug delivery (SDD) mediated through alternating magnetic fields (AMF). In this work, magnesium ferrite (MgFeO) encapsulated with liposomes lipid bilayer (MLs), Quercetin (Q)-loaded MgFeO@Liposomes (Q-MLs) nano-hybrid system were successfully synthesized for magnetic hyperthermia (MHT) and SDD applications. The hybrid system was well-investigated by different techniques using X-ray diffraction (XRD), Fourier transforms infrared spectroscopy (FT-IR), Energy dispersive X-ray (EDX), Vibrating sample magnetometer (VSM), Transmission electron microscope (TEM), and Zeta Potential (ZP). The characterization results confirmed the improving quercetin-loading on the MLs surface. TEM analysis indicated the synthesized MgFeO, MLs, and Q-MLs were spherical with an average size of 23.7, 35.5, and 329.5 nm, respectively. The VSM results revealed that the MgFeO exhibit excellent and effective saturation magnetization (M) (40.5 emu/g). Quercetin drug loading and entrapment efficiency were found to be equal to 2.1 ± 0.1% and 42.3 ± 2.2%, respectively. The in-vitro Q release from Q-loaded MLs was found 40.2% at pH 5.1 and 69.87% at pH 7.4, verifying the Q-loading pH sensitivity. The MLs and Q-MLs hybrid system as MHT agents exhibit specific absorption rate (SAR) values of 197 and 205 W/g, correspondingly. Furthermore, the Q-MLs cytotoxicity was studied on the MCF-7 breast cancer cell line, and the obtained data demonstrated that the Q-MLs have a high cytotoxicity effect compared to MLs and free Q.
新型生物相容性和有效的热疗（HT）治疗材料最近引起了研究人员的关注，因为它们可以有效消融癌细胞，而对健康细胞的损伤可以忽略不计。磁脂质体（MLs）在癌症治疗中有许多应用的可能性，包括通过交变磁场（AMF）进行智能药物输送（SDD）。在这项工作中，成功合成了脂质体双层包裹的镁铁氧体（MgFeO）（MLs）、负载姜黄素（Q）的 MgFeO@Liposomes（Q-MLs）纳米杂化系统，用于磁热疗（MHT）和 SDD 应用。该杂化系统通过 X 射线衍射（XRD）、傅里叶变换红外光谱（FT-IR）、能谱（EDX）、振动样品磁强计（VSM）、透射电子显微镜（TEM）和 Zeta 电位（ZP）等不同技术进行了详细研究。表征结果证实了 MLs 表面姜黄素载量的提高。TEM 分析表明，合成的 MgFeO、MLs 和 Q-MLs 分别为球形，平均粒径为 23.7、35.5 和 329.5nm。VSM 结果表明，MgFeO 表现出优异的有效饱和磁化强度（M）（40.5emu/g）。姜黄素的载药量和包封效率分别为 2.1±0.1%和 42.3±2.2%。在 pH5.1 时，负载姜黄素的 MLs 的体外 Q 释放量为 40.2%，在 pH7.4 时为 69.87%，验证了 Q 载药的 pH 敏感性。作为 MHT 剂的 MLs 和 Q-MLs 杂化系统的比吸收率（SAR）值分别为 197 和 205W/g。此外，还研究了 Q-MLs 在 MCF-7 乳腺癌细胞系上的细胞毒性，所得数据表明，与 MLs 和游离 Q 相比，Q-MLs 具有更高的细胞毒性作用。
