Shah V C, Mittal S C
Strahlenther Onkol. 1987 Jan;163(1):54-60.
Effect of different doses of gamma-irradiation on SCE induction in unifiliarly 5-bromo 2-deoxyuridine substituted DNA was studied in various phases of cell cycle. Changes in gamma-irradiation induced SCE frequency was measured by post-irradiation treatment with antimutagen L-cysteine. Perturbation in cellular proliferation kinetics due to gamma-irradiation and gamma-irradiation plus L-cysteine was also studied. It was observed that gamma-irradiation is an efficient inducer of SCE and is most effective in S phase. L-cysteine also causes SCE induction which is slightly higher than the spontaneous level of SCEs found in HeLa cells. However, post-irradiation addition of L-cysteine reduces SCE frequency in gamma-irradiated cultures and this reduction is maximum in G1 phase irradiated cells. gamma-irradiation delayed the mitosis considerably and this delay continued to increase with increasing doses. L-cysteine reduced the delay in cell cycle caused by gamma-irradiation.
研究了不同剂量的γ射线照射对单链5-溴-2-脱氧尿苷取代DNA中姐妹染色单体交换（SCE）诱导的影响，该研究在细胞周期的各个阶段进行。通过用抗诱变剂L-半胱氨酸进行辐照后处理，测量γ射线照射诱导的SCE频率变化。还研究了γ射线照射以及γ射线照射加L-半胱氨酸对细胞增殖动力学的干扰。观察到γ射线照射是SCE的有效诱导剂，并且在S期最有效。L-半胱氨酸也会引起SCE诱导，其略高于HeLa细胞中SCE的自发水平。然而，辐照后添加L-半胱氨酸会降低γ射线照射培养物中的SCE频率，并且这种降低在G1期照射的细胞中最大。γ射线照射显著延迟了有丝分裂，并且这种延迟随着剂量的增加而持续增加。L-半胱氨酸减少了γ射线照射引起的细胞周期延迟。
