经颅聚焦超声调节小鼠的心肺活动。
Modulation of cardio-respiratory activity in mice via transcranial focused ultrasound.
机构信息
Department of Biomedical Engineering, Columbia University, 630 West 168th Street, P&S 19-419, New York, NY, 10032, United States.
Department of Biomedical Engineering, Columbia University, 630 West 168th Street, P&S 19-419, New York, NY, 10032, United States; Department of Radiology, Columbia University, 630 West 168th Street, P&S 19-419, New York, NY, 10032, United States.
出版信息
Ultrasound Med Biol. 2024 Mar;50(3):332-340. doi: 10.1016/j.ultrasmedbio.2023.11.003. Epub 2023 Dec 17.
OBJECTIVE
The objective of this study was to investigate the effect of FUS on autonomic nervous system activity, including heart and respiratory rates, and to separate the thermal modulation from combined thermal and mechanical FUS effects.
METHODS
The thalamus and hypothalamus of wild-type mice were sonicated with a continuous-wave, 2 MHz FUS transducer at pressures of 425 and 850 kPa for 60 seconds. Cardiac and respiratory rates were monitored as signs of autonomic nervous activity. FUS-induced changes in autonomic activity were compared to FUS targeted to a spatially-distant motor region and to laser-induced heating.
RESULTS
FUS delivered to the primary target over the thalamus and hypothalamus at 850 kPa reversibly increased the respiratory rate by 6.5±3.2 breaths per minute and decreased the heart rate by 3.2±1.8 beats per minute. No significant changes occurred in this region at 425 kPa or when targeting the motor regions at 850 kPa. Laser heating with the same temperature rise profile produced by 850 kPa sonication resulted in cardiorespiratory modulation similar to that of FUS.
CONCLUSIONS
FUS is capable of reversibly and non-invasively modulating cardiorespiratory activity in mice. Localized changes in temperature may constitute the main cause for this activity, though further investigation is warranted into the distinct and complementary mechanisms of mechanically- and thermally-induced FUS neuromodulation. Close monitoring of vital signs during FUS neuromodulation may be warranted to monitor systemic responses to stimulation.
目的
本研究旨在探讨 FUS 对自主神经系统活动（包括心率和呼吸率）的影响，并将热调节与热和机械联合 FUS 效应分开。
方法
使用连续波、2MHz FUS 换能器在 425kPa 和 850kPa 的压力下对野生型小鼠的丘脑和下丘脑进行超声处理，持续 60 秒。监测心率和呼吸率作为自主神经活动的标志。将 FUS 引起的自主活动变化与 FUS 靶向空间上分离的运动区域以及激光诱导加热进行比较。
结果
在 850kPa 时，将 FUS 传递到丘脑和下丘脑的主要靶位，可使呼吸频率可逆性增加 6.5±3.2 次/分钟，使心率降低 3.2±1.8 次/分钟。在 425kPa 或在 850kPa 时靶向运动区域时，该区域没有发生显著变化。以 850kPa 声辐射产生相同温升曲线的激光加热导致心肺调节类似于 FUS。
结论
FUS 能够可逆地、非侵入性地调节小鼠的心肺活动。局部温度变化可能是这种活动的主要原因，但需要进一步研究机械和热诱导 FUS 神经调节的独特和互补机制。在 FUS 神经调节期间密切监测生命体征可能需要监测刺激的全身反应。
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