Haley N J, Adams J D, Alzofon J, Hoffmann D
Toxicol Lett. 1987 Jan;35(1):83-8. doi: 10.1016/0378-4274(87)90089-0.
An inhalation bioassay with Syrian golden hamsters is being conducted to evaluate the toxic and carcinogenic potential of cigarette sidestream smoke (SS) relative to mainstream smoke (MS). A Hamburg II smoking machine is used to deliver MS by nose-only exposure to hamsters and a modification allows for the simultaneous collection of SS for whole-body delivery to a different rack of animals. The tolerated dose of SS was determined by varying the air/smoke dilutions drawn through the animal restrainers. Preliminary data indicated that 20% carboxyhemoglobin (COHb) could be obtained in SS-exposed animals without fatality. Optimum exposure levels were determined. Monthly measurements of COHb, nicotine and cotinine indicate that the SS-exposed animals are absorbing slightly higher amounts of these smoke constituents than the MS-exposed hamsters. Tumor incidence and carcinogenicity data are being collected through complete necropsy and histology protocols and uptake data continue to be collected. These studies should help elucidate the carcinogenic potential of SS which has been suggested from its composition and from recent epidemiological data of cancer incidence in non-smokers.
正在用叙利亚金黄地鼠进行一项吸入生物测定，以评估香烟侧流烟雾（SS）相对于主流烟雾（MS）的毒性和致癌潜力。使用一台汉堡II型吸烟机通过仅经鼻暴露的方式向地鼠输送主流烟雾，并且经过改装后可以同时收集侧流烟雾，以便将其全身输送给另一组笼子里的动物。通过改变通过动物固定器抽取的空气/烟雾稀释度来确定侧流烟雾的耐受剂量。初步数据表明，暴露于侧流烟雾的动物能够达到20%的碳氧血红蛋白（COHb）水平而无死亡。确定了最佳暴露水平。每月对碳氧血红蛋白、尼古丁和可替宁的测量表明，暴露于侧流烟雾的动物比暴露于主流烟雾的地鼠吸收的这些烟雾成分略多。正在通过完整的尸检和组织学方案收集肿瘤发生率和致癌性数据，并且继续收集摄取数据。这些研究应有助于阐明侧流烟雾的致癌潜力，这一点已从其成分以及近期非吸烟者癌症发病率的流行病学数据中得到暗示。
