Griffith R B, Standafer S
Toxicology. 1985 Apr;35(1):13-24. doi: 10.1016/0300-483x(85)90128-3.
A system for exposing rats to mainstream (MS) and sidestream (SS) smoke simultaneously from the same cigarette, and monitoring procedures, are described in detail. The equipment and procedures were used to expose Sprague-Dawley rats to mainstream smoke and to target deliveries of 10, 25, or 50% of the total SS smoke for 17 weeks. The estimated total particulate matter (TPM) dose was highly correlated with the increase in percent COHb for MS and SS smoke, but the COHb/TPM relationships were different for the 2 kinds of smoke. All SS TPM doses were much lower than the MS TPM dose, and the COHb/TPM ratio for SS smoke was much higher than for MS smoke. The TPM dose and per cent COHb for SS smoke were highly correlated with the per cent of SS sent to the exposure chambers. There were no significant differences in the total weight changes during the study for any of the smoke exposed groups, but weight changes during the 12-17-week period for sidestream exposed groups were inversely correlated with the level of sidestream exposure.
详细描述了一种使大鼠同时暴露于同一支香烟的主流（MS）烟雾和侧流（SS）烟雾中的系统以及监测程序。该设备和程序用于使斯普拉格-道利大鼠暴露于主流烟雾，并使其暴露于占总侧流烟雾10%、25%或50%的目标剂量下，持续17周。估计的总颗粒物（TPM）剂量与主流烟雾和侧流烟雾中碳氧血红蛋白（COHb）百分比的增加高度相关，但两种烟雾的COHb/TPM关系有所不同。所有侧流TPM剂量均远低于主流TPM剂量，且侧流烟雾的COHb/TPM比值远高于主流烟雾。侧流烟雾的TPM剂量和COHb百分比与输送到暴露舱的侧流烟雾百分比高度相关。在研究期间，任何烟雾暴露组的总体重变化均无显著差异，但侧流暴露组在第12至17周期间的体重变化与侧流暴露水平呈负相关。
