Institute of Tropical Agriculture and Forestry, Hainan University, Hainan, PR China.
One Health Institute, Hainan University, Hainan, PR China.
Vet Med Sci. 2024 Jan;10(1):e1339. doi: 10.1002/vms3.1339. Epub 2023 Dec 18.
Endothelial cell migration is the initial stage of angiogenesis. In previous studies, miR-9 has been found to regulate angiogenesis and cell migration in human medicine.
This study aimed to reveal the regulatory effect of miR-9 on canine endothelial cell migration.
Embryonic canine ventricle myocardium tissues were collected and induced to differentiate into endothelial-like cells (ELCs). A transwell and invasion assay were used to evaluate the impact of miR-9 on the migration capacity of ELCs, after which a luciferase reporter assay, western blotting, RNA sequencing and reverse transcription-polymerase chain reaction were conducted to explore the regulatory mechanism.
Our results showed that we successfully induced the primary cells derived from canine cardiac embryo tissues into ELCs. MiR-9 also promoted the migration and invasion of canine ELCs, and inhibited the expression of collagen XV, an angiogenic inhibitor, at the translational level by targeting the 3' untranslated region of COL15A1 gene. Furthermore, RNA sequencing showed that overexpression of miR-9 impacted several signalling pathways and eight genes involved in angiogenesis and cell migration in canine ELCs.
These findings suggest that miR-9 enhances the migration of canine ELCs and may serve as a potential diagnostic and therapeutic target for canine diseases involved in endothelial cells migration and angiogenesis, but more further studies are needed.
内皮细胞迁移是血管生成的初始阶段。在之前的研究中，miR-9 被发现可调节人类医学中的血管生成和细胞迁移。
本研究旨在揭示 miR-9 对犬内皮细胞迁移的调节作用。
采集胚胎犬心室心肌组织并诱导分化为内皮样细胞（ELC）。通过 Transwell 和侵袭实验评估 miR-9 对 ELC 迁移能力的影响，然后通过荧光素酶报告基因实验、Western blot、RNA 测序和逆转录-聚合酶链反应探索其调控机制。
我们的结果表明，我们成功地将源自犬心脏胚胎组织的原代细胞诱导分化为 ELC。miR-9 还促进了犬 ELC 的迁移和侵袭，并通过靶向 COL15A1 基因的 3'非翻译区抑制了血管生成抑制剂胶原 XV 的翻译表达。此外，RNA 测序显示 miR-9 的过表达影响了犬 ELC 中几个参与血管生成和细胞迁移的信号通路和 8 个基因。
这些发现表明 miR-9 增强了犬 ELC 的迁移，可能成为涉及内皮细胞迁移和血管生成的犬疾病的潜在诊断和治疗靶点，但需要进一步研究。
