Korolev V G, Ivanov E L
Genetika. 1979 Jun;15(6):1024-32.
The lethal effect of 35S and 32P decays on cells of yeast radiation-sensitive mutant xrs2 was studied. The mutant is 7 times more sensitive than the wild type to transmutation of both isotopes. The survival curve for xrs2 was exponential. In spite of the lethal effect, mutant cells are not more mutable than the wild type under decays of both isotopes (the number of mutations in ade1 and ade2 genes was counted), xrs2 and wild type strains differ in kinds of mutations induced by the decay of incorporated 35S in ade2 locus. Namely, there are 82% of base substitutions and 18% of other types mutations induced in xrs2 strain despite 97% and 3% respectively for the wild type strain. Also it was shown that complete and mosaic mutants, induced by the the 35S decay in xrs2 strain, differ in a pattern of interallelic complementation.
研究了35S和32P衰变对酵母辐射敏感突变体xrs2细胞的致死效应。该突变体对两种同位素的嬗变比野生型敏感7倍。xrs2的存活曲线呈指数形式。尽管存在致死效应，但在两种同位素衰变情况下，突变体细胞的可突变性并不比野生型更高（计算了ade1和ade2基因中的突变数），xrs2和野生型菌株在ade2基因座中掺入的35S衰变诱导的突变类型上存在差异。具体而言，xrs2菌株中诱导的碱基替换占82%，其他类型突变占18%，而野生型菌株分别为97%和3%。还表明，xrs2菌株中由35S衰变诱导的完全突变体和镶嵌突变体在等位基因间互补模式上存在差异。
