Department of Women's and Children's Health, Division of Neurology/Pediatric Orthopedics, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden.
Institute of Human Movement Science, Sport and Health, University of Graz, Austria.
Dev Med Child Neurol. 2024 Jul;66(7):902-909. doi: 10.1111/dmcn.15835. Epub 2023 Dec 18.
The aim of this observational study was to determine the immune status and function in young adults with cerebral palsy (CP) in comparison to typically developing individuals.
Blood samples from 12 individuals with CP (five males, seven females; mean age: 25 years 1 month (5 years 9 months); age range: 19-38 years) and 17 typically developing individuals (eight males, nine females; mean age: 31 years 4 months (6 years 2 months); age range: 20-40 years) were collected before, immediately after, and 1 hour after 45 minutes of frame running or running respectively. Independent t-tests were used to compare heart rate, level of exertion, and baseline cell proportions between groups. Mixed model analysis of variance was utilized to investigate immune cell responses to exercise across groups.
Baseline levels of gamma delta (TCRγδ+) T-cells were significantly higher (absolute percentage: +2.65, p = 0.028) in the individuals with CP. Several cell populations showed similar significant changes after exercise in both CP and typically developing groups. Cytotoxic (CD8+) T-cells were only significantly elevated immediately after exercise in the typically developing participants (p < 0.01). Individuals with CP exhibited significantly lower heart rates (-11.1%, p < 0.01), despite similar ratings of perceived exertion.
Elevated baseline TCRγδ+ T-cells may indicate low-grade inflammation in adults with CP. Although most of the cell populations showed typical responses to endurance exercise, the absence of response in CD8+ T-cells in individuals with CP may indicate the need for higher intensity during exercise.
TCRγδ+ T-cell baseline levels are elevated in adults with cerebral palsy (CP). The CD8+ T-cell response to exercise was blunted in adults with CP. Exercise intensity is decisive for CD8+ T-cell responses in individuals with CP.
本观察性研究旨在比较脑瘫（CP）患者与正常发育个体的免疫状态和功能。
采集 12 例 CP 患者（5 例男性，7 例女性；平均年龄：25 岁 1 个月（5 年 9 个月）；年龄范围：19-38 岁）和 17 例正常发育个体（8 例男性，9 例女性；平均年龄：31 岁 4 个月（6 年 2 个月）；年龄范围：20-40 岁）的血液样本，分别在框式跑步或跑步 45 分钟前后即刻及 1 小时后采集。独立 t 检验用于比较两组间心率、运动强度和基线细胞比例。混合模型方差分析用于研究免疫细胞对运动的反应。
CP 患者的γδ（TCRγδ+）T 细胞基线水平显著升高（绝对值：+2.65，p=0.028）。CP 和正常发育组在运动后均有几个细胞群出现类似的显著变化。运动后即刻，仅正常发育组的细胞毒性（CD8+）T 细胞显著升高（p<0.01）。CP 患者的心率显著降低（-11.1%，p<0.01），尽管他们的感觉运动强度相似。
CP 患者 TCRγδ+T 细胞基线水平升高可能表明其存在低度炎症。尽管大多数细胞群对耐力运动表现出典型反应，但 CP 患者 CD8+T 细胞无反应可能表明运动时需要更高强度。
CP 患者的 TCRγδ+T 细胞基线水平升高。CP 患者的 CD8+T 细胞对运动的反应减弱。运动强度对 CP 患者 CD8+T 细胞反应起决定性作用。
