Qin Gaofeng, Song Rongqiang, Sun Jingyi, Chen Bing, Liu Zhe, Han Lei, Sun Baoliang, Li Chen
Shandong University of Traditional Chinese Medicine, Jinan, Shangdong China.
Department of Traditional Chinese Medicine, Binzhou Medical University Hospital, Binzhou, 256603 Shandong China.
3 Biotech. 2025 Jan;15(1):14. doi: 10.1007/s13205-024-04181-6. Epub 2024 Dec 17.
There is currently no effective treatment for Alzheimer's disease (AD). This research explored Shenzhiling Oral Liquid (SZLD) against AD by pinpointing crucial elements and understanding its molecular mechanisms through network pharmacology and in vitro experiment. First, we used network pharmacology to screen the main targets and mechanisms of SZLD to improve AD. Then we conducted experiments with Aβ42-induced SH-SY5Y cells to assess SZLD's impact, focusing particularly on apoptotic pathways, thereby uncovering its mechanism of action in AD. Through our analysis, we discovered a notable link between SZLD's effect on AD and apoptosis processes. Specifically, the critical proteins Casapse3 and BCL-2 showed strong correlations in this context. Through systematic data analysis and experimental verification, we unveiled the healing advantages and the foundational molecular mechanisms of SZLD in AD. These findings underscore the promising and compelling potential of targeting the PI3K/Akt signaling pathway and apoptosis with SZLD as a therapeutic strategy to ameliorate AD.
目前尚无治疗阿尔茨海默病（AD）的有效方法。本研究通过网络药理学和体外实验，找出关键因素并了解其分子机制，探索了参知灵口服液（SZLD）治疗AD的作用。首先，我们运用网络药理学筛选出SZLD改善AD的主要靶点和机制。然后，我们用Aβ42诱导的SH-SY5Y细胞进行实验，评估SZLD的影响，特别关注凋亡途径，从而揭示其在AD中的作用机制。通过分析，我们发现SZLD对AD的作用与凋亡过程之间存在显著联系。具体而言，关键蛋白半胱天冬酶3（Casapse3）和B细胞淋巴瘤-2（BCL-2）在这种情况下显示出很强的相关性。通过系统的数据分析和实验验证，我们揭示了SZLD在AD治疗中的优势和基础分子机制。这些发现强调了以PI3K/Akt信号通路和凋亡为靶点，将SZLD作为改善AD的治疗策略具有广阔且引人注目的潜力。
