Stoianov T, Zagorski D
Vet Med Nauki. 1986;23(9):69-76.
Investigations were carried out to test the effect of various temperature regimes of thawing (40 degrees, 50 degrees, 60 degrees, 70 degrees C) of bull semen frozen after the method of Nagase-Niwa (1964) in the form of pellets sizing 0.15 to 0.20 cm3. It was found that with the rise of water bath temperature the number of restored spermatozoa after thawing also rose. The higher temperatures of thawing led to a reliable shortening of the time of exposure needed for the thawing of individual pellets. Tested was a method and a device for the more effective and safe thawing of semen at higher temperatures. Artificial insemination carried out with semen thawing after the new method--55 degrees C--made it possible to raise the conception rate of the first service by 5.39 per cent as compared to the traditionally employed technology of thawing--38 degrees--40 degrees C--in cattle breeding practice.
开展了多项研究，以测试按照长濑-丹羽（1964年）的方法冻成0.15至0.20立方厘米丸粒状的公牛精液，在不同解冻温度条件（40摄氏度、50摄氏度、60摄氏度、70摄氏度）下的解冻效果。结果发现，随着水浴温度升高，解冻后恢复活力的精子数量也增加。较高的解冻温度能可靠地缩短单个丸粒的解冻所需时间。测试了一种在较高温度下更有效且安全地解冻精液的方法和装置。在养牛实践中，采用新方法（55摄氏度）解冻精液进行人工授精，与传统采用的38至40摄氏度解冻技术相比，首次输精的受胎率提高了5.39%。
