Stoianov T, Zagorski D
Vet Med Nauki. 1987;24(8):91-5.
Laboratory and biologic methods of investigation were employed to produce and test a new medium for thawing pelleted semen of breeding bulls. The composition of the medium included glucose, sodium nitrate, sodium bicarbonate, potassium chloride, ascorbic acid, nicotinic acid, amino acetic acid, and gentamycin sulfate. Thawed spermatozoa in this medium showed dependably higher motility, heat resistance, and oxygen consumption as against spermatozoa kept in a control medium. Semen thawed in the newly produced medium was used to inseminate a total of 3,142 cows, whereupon the fertility rate at first insemination was 9 per cent higher than that of the control cows.
采用实验室和生物学研究方法来制备和测试一种用于解冻种公牛颗粒精液的新培养基。该培养基的成分包括葡萄糖、硝酸钠、碳酸氢钠、氯化钾、抗坏血酸、烟酸、氨基乙酸和硫酸庆大霉素。与保存在对照培养基中的精子相比，在这种培养基中解冻的精子表现出更高的活力、耐热性和耗氧量。在新制备的培养基中解冻的精液用于对总共3142头母牛进行授精，首次授精时的受胎率比对照母牛高9%。
