Simon A E, Howell S H
Virology. 1987 Jan;156(1):146-52. doi: 10.1016/0042-6822(87)90445-4.
RNA copies, synthesized in vitro, of the virulent satellite (RNA C) of turnip crinkle virus (TCV) infect plants when coinoculated with helper virus RNA. RNA C is a small linear RNA of about 355 bases which intensifies TCV symptoms in infected plants. Full-length cDNA copies of the satellite were inserted in an expression vector (for RNA synthesis in vitro) in such a way that RNA synthesized in vitro had the same 5'-end as the native satellite. Plus-strand RNA copies of the satellite in near-monomer and multimer form infected plants, while minus-strand RNA copies and DNA copies of the satellite RNA did not do so under the conditions tested. When plants were inoculated with RNAs synthesized in vitro from two independently cloned satellite cDNAs with base sequence and length differences, the products of infection corresponded in sequence to the different cRNAs used in the inocula. Satellite RNAs synthesized in vitro from either cDNA produced the same symptoms as the native satellite RNA.
芜菁皱缩病毒（TCV）强毒卫星（RNA C）的体外合成RNA拷贝与辅助病毒RNA共接种时可感染植物。RNA C是一种约355个碱基的小线性RNA，可加剧受感染植物中的TCV症状。将卫星的全长cDNA拷贝插入表达载体（用于体外RNA合成），使得体外合成的RNA具有与天然卫星相同的5'末端。近单体和多聚体形式的卫星正链RNA拷贝感染植物，而卫星RNA的负链RNA拷贝和DNA拷贝在测试条件下则不能感染。当用从两个具有碱基序列和长度差异的独立克隆的卫星cDNA体外合成的RNA接种植物时，感染产物的序列与接种物中使用的不同cRNA一致。从任一cDNA体外合成的卫星RNA产生与天然卫星RNA相同的症状。
