Zhang C X, Cascone P J, Simon A E
Molecular and Cellular Biology Program, University of Massachusetts, Amherst 01003.
Virology. 1991 Oct;184(2):791-4. doi: 10.1016/0042-6822(91)90454-j.
New recombinant molecules formed from satellite and genomic RNAs of turnip crinkle virus (TCV) have been characterized. Known collectively as sat-RNA CX, these molecules are composed of a nearly full-length segment of a previously characterized TCV satellite RNA (sat-RNA D) at the 5' end joined to variable lengths of TCV genomic RNA 3' terminal sequence. Sat-RNA CX molecules fall into two classes: molecules of 420 to 435 bases and larger species of 501 to 506 bases. The TCV sequence at the junction of the larger molecules is purine-rich and is similar to a motif found at the 5' ends of the TCV satellite RNAs and at the junctions of some TCV defective interfering RNAs. The TCV sequence at the junction of the smaller sat-RNA CX molecules is pyrimidine-rich and is similar to the sequence at the right side of a junction of one TCV defective interfering RNA as well as sequence immediately downstream of the internal initiation site of the 1.45-kb TCV subgenomic RNA. We propose that the latter motif is another putative signal recognized by the viral replicase during the generation of defective interfering and recombinant RNAs in the TCV system.
已对由芜菁皱缩病毒（TCV）的卫星RNA和基因组RNA形成的新型重组分子进行了表征。这些分子统称为sat-RNA CX，由5'端一个先前已表征的TCV卫星RNA（sat-RNA D）的近乎全长片段与不同长度的TCV基因组RNA 3'末端序列连接而成。Sat-RNA CX分子分为两类：420至435个碱基的分子和501至506个碱基的更大种类。较大分子连接处的TCV序列富含嘌呤，类似于在TCV卫星RNA的5'端以及一些TCV缺陷干扰RNA的连接处发现的基序。较小的sat-RNA CX分子连接处的TCV序列富含嘧啶，类似于一种TCV缺陷干扰RNA连接处右侧的序列以及1.45-kb TCV亚基因组RNA内部起始位点下游紧邻的序列。我们提出，后一种基序是病毒复制酶在TCV系统中产生缺陷干扰RNA和重组RNA过程中识别的另一个假定信号。
