Pizarro F, Amar M, Stekel A
Am J Clin Nutr. 1987 Feb;45(2):484-7. doi: 10.1093/ajcn/45.2.484.
We describe a quantitative method for determination of iron in stool to monitor consumption of iron-fortified milk in infants. The method is simple, fast, and inexpensive. Stool samples from infants consuming fortified milk or nonfortified milk were ashed, and ashes were diluted in hydrochloric acid and reacted with bathophenantroline disulphonate. Mean iron excretion per subject was obtained. Anemia was present in 25.7% of infants in the nonfortified group (upper level of 95% confidence limit for mean iron excretion was 14.9 mg iron/100 g stool) and in 22.2% of infants from the noncompliant fortified group (mean excretion less than 15 mg iron/100 g stool). In contrast, only 0.8% of infants who had properly consumed the fortified milk had anemia. We conclude that this method for determination of stool iron should be considered for estimating compliance in evaluation of results of field trials of iron-fortified products in infants.
我们描述了一种定量测定粪便中铁含量的方法，用于监测婴儿对铁强化牛奶的摄入量。该方法简单、快速且成本低廉。采集食用强化牛奶或非强化牛奶婴儿的粪便样本进行灰化，将灰分用盐酸稀释后与4,7-二苯基-1,10-菲啰啉二磺酸反应，得出每位受试者的平均铁排泄量。非强化组25.7%的婴儿存在贫血（平均铁排泄量的95%置信上限为14.9毫克铁/100克粪便），未达标准的强化组中22.2%的婴儿存在贫血（平均排泄量低于15毫克铁/100克粪便）。相比之下，正确食用强化牛奶的婴儿中只有0.8%患有贫血。我们得出结论，在评估婴儿铁强化产品的现场试验结果时，应考虑使用这种测定粪便中铁含量的方法来评估依从性。
