Clinic of Gynecology and Obstetrics, Sestre milosrdnice University Hospital Center, Zagreb, Croatia.
Department of Clinical Chemistry, Sestre milosrdnice University Hospital Center, Zagreb, Croatia.
Biochem Med (Zagreb). 2024 Feb 15;34(1):030701. doi: 10.11613/BM.2024.010701. Epub 2023 Dec 15.
Paraoxonase 1 (PON1) is the enzyme that removes carcinogenic radicals from lipids. The aim of the study was to investigate the differences in PON1 activity and oxidation stress parameters between patients with cervical intraepithelial neoplasia (CIN) and healthy controls.
The study included 65 women with CIN and 109 healthy women. Lipid parameters were determined on Cobas Integra 400 plus (Roche, Mannheim, Germany). Tiols and reduced glutathione (GSH) were determined spectrophotometric using Eliman reagent. Activity of PON1 was assessed with two substrates, paraoxon and phenylacetate by spectrophotometric method. Malondialdehyde (MDA) was determined by high performance liquid chromatography (Shimadzu Corporation, Kyoto, Japan). Mann-Whitney-test, t-test, χ2-test, correlation and logistic regression was used in statistical analysis. P < 0.05 was considered statistically significant.
The basal (P = 0.929) and NaCl-stimulated (P = 0.985) PON1 activity and activities standardised on the concentration of high-density lipoprotein (HDL; P = 0.076; P = 0.065, respectively) and apolipoprotein AI (apo AI; P = 0.444; P = 0.499, respectively) as well as PON1 phenotypes (P = 0.842) did not differ significantly between the groups. The PON1 arylesterase activity (53±19 kU/L vs. 77±17 kU/L; P < 0.001) and HDL-standardized activity (37 (28-44) kU/mmol . 43 (37-50) kU/mmol; P < 0.001) and apoAI (29±11 kU/g . 44±11 kU/g; P < 0.001) was significantly reduced in the CIN group. The concentration of the thiol groups was similar (P = 0.519), of MDA was lower (0.39 (0.27-0.55) µmol/L . 0.76 (0.57-1.15) µmol/L; P < 0.001) and of GSH was higher (112.0 (66.0-129.6) µg/mL . 53.4 (34.8-134.4) µg/mL; P < 0.001) in the CIN group.
Reduced PON1 arylesterase activity, lower MDA and higher GSH concentration were observed in CIN patients.
对氧磷酶 1（PON1）是一种从脂质中去除致癌自由基的酶。本研究旨在探讨宫颈癌前病变（CIN）患者与健康对照者之间 PON1 活性和氧化应激参数的差异。
本研究纳入了 65 例 CIN 患者和 109 例健康女性。采用 Cobas Integra 400 plus（罗氏，曼海姆，德国）测定脂质参数。采用 Eliman 试剂比色法测定巯基和还原型谷胱甘肽（GSH）。采用分光光度法，用对氧磷和苯乙酸两种底物评估 PON1 活性。采用高效液相色谱法（岛津公司，京都，日本）测定丙二醛（MDA）。统计分析采用曼-惠特尼检验、t 检验、卡方检验、相关性和逻辑回归。P<0.05 被认为具有统计学意义。
基础（P=0.929）和 NaCl 刺激（P=0.985）的 PON1 活性以及高密度脂蛋白（HDL）浓度标准化（P=0.076；P=0.065）和载脂蛋白 AI（apo AI；P=0.444；P=0.499）的 PON1 活性以及 PON1 表型（P=0.842）在两组间无显著差异。CIN 组的 PON1 芳基酯酶活性（53±19 kU/L vs. 77±17 kU/L；P<0.001）和 HDL 标准化活性（37（28-44）kU/mmol vs. 43（37-50）kU/mmol；P<0.001）和 apoAI（29±11 kU/g vs. 44±11 kU/g；P<0.001）显著降低。巯基浓度相似（P=0.519），丙二醛浓度较低（0.39（0.27-0.55）µmol/L vs. 0.76（0.57-1.15）µmol/L；P<0.001），GSH 浓度较高（112.0（66.0-129.6）µg/mL vs. 53.4（34.8-134.4）µg/mL；P<0.001）在 CIN 组中。
在 CIN 患者中观察到 PON1 芳基酯酶活性降低、MDA 降低和 GSH 浓度升高。
