University of Rome "Tor Vergata", Department of Chemical Science and Technologies, Via della Ricerca Scientifica, 00133 Rome, Italy.
University of Rome "Tor Vergata", Department of Chemical Science and Technologies, Via della Ricerca Scientifica, 00133 Rome, Italy; SENSE4MED, Via Bitonto 139, 00133, Rome, Italy.
Bioelectrochemistry. 2024 Apr;156:108619. doi: 10.1016/j.bioelechem.2023.108619. Epub 2023 Nov 29.
Herein, we developed a new waste solution-free paper-based electrochemical immunosensor for SARS-CoV-2 detection in saliva, by combining vertical and lateral flow. In detail, the device was constituted of a reservoir containing all reagents for the construction of the immunological chain onto the magnetic beads and a lateral flow holder which contained a polyester-based electrode, a magnet, and an adsorbent pad. The measurement was carried out by adding the saliva sample into the reservoir, followed by the addition of this solution in the hole present in the lateral flow holder. The successive additions of washing buffer and TMB solution in the lateral flow holder allowed the detection of N protein in saliva in the range of 0.06 to 4 µg/mL with a detection limit equal to 30 ng/mL. The analysis of several saliva samples with the sensing tool and the reference method, demonstrated the effectiveness of this device, being able to identify positive patients with high values of CT e.g. 35. This new configuration paves the way for the realization of any magnetic beads-based immunosystem without waste solution production, enlarging the application of paper-based devices.
在此，我们开发了一种新的无废液基于纸的电化学免疫传感器，用于唾液中 SARS-CoV-2 的检测，结合了垂直和横向流动。具体来说，该装置由一个包含所有用于将免疫链构建到磁性珠上的试剂的储液器和一个包含聚酯基电极、磁铁和吸附垫的横向流动支架组成。测量是通过将唾液样本加入储液器中，然后将该溶液加入横向流动支架上的孔中进行的。在横向流动支架中连续加入洗涤缓冲液和 TMB 溶液，允许在 0.06 至 4 µg/mL 的范围内检测唾液中的 N 蛋白，检测限等于 30 ng/mL。使用传感工具和参考方法对几个唾液样本进行分析，证明了该设备的有效性，能够识别 CT 值较高的阳性患者，例如 35。这种新配置为实现任何无废液产生的基于磁性珠的免疫分析系统铺平了道路，扩大了基于纸张的设备的应用。
