Sokoloski J A, Jastreboff M M, Bertino J R, Sartorelli A C, Narayanan R
Anal Biochem. 1986 Nov 1;158(2):272-7. doi: 10.1016/0003-2697(86)90549-x.
A simple method, employing high-voltage electric discharge (electroporation), was developed to introduce phosphorylated nucleosides into the cytoplasm of viable cells. HL-60 leukemia cells permeabilized by this technique remained viable and incorporated deoxyribonucleoside triphosphates into nuclear DNA. Furthermore, DNA synthesis was depressed for at least 24 h in HL-60 cells made permeable to 1-beta-D-arabinosylcytosine 5'-triphosphate by this methodology. Electroporation was found to be applicable to the permeabilization of a wide variety of cell lines in culture to nucleotides, suggesting that this methodology may be useful for the introduction into intact cells of a wide variety of molecules that are not normally transported effectively.
一种采用高压放电（电穿孔）的简单方法被开发出来，用于将磷酸化核苷导入活细胞的细胞质中。通过该技术通透化的HL-60白血病细胞仍保持存活，并将脱氧核糖核苷三磷酸掺入核DNA中。此外，通过该方法使对1-β-D-阿拉伯糖基胞嘧啶5'-三磷酸通透的HL-60细胞中，DNA合成至少被抑制24小时。发现电穿孔适用于使培养中的多种细胞系对核苷酸通透化，这表明该方法可能有助于将多种通常不能有效转运的分子导入完整细胞。
