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通过电穿孔将外源DNA导入莱茵衣藻。

Introduction of exogenous DNA into Chlamydomonas reinhardtii by electroporation.

作者信息

Brown L E, Sprecher S L, Keller L R

机构信息

Department of Biological Science, Florida State University, Tallahassee 32306.

出版信息

Mol Cell Biol. 1991 Apr;11(4):2328-32. doi: 10.1128/mcb.11.4.2328-2332.1991.

DOI:10.1128/mcb.11.4.2328-2332.1991
PMID:2005916
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC359944/
Abstract

The fate of exogenous DNA introduced into Chlamydomonas reinhardtii by electroporation was analyzed. With single and double electrical pulses, plasmids as large as 14 kb were introduced into cells with and without intact cell walls. Within hours after introduction, exogenous plasmid DNA was associated with nuclei isolated from cells; several weeks after introduction, exogenous DNA was stably integrated into the Chlamydomonas genome. These studies establish electroporation as a method for introducing DNA, and potentially other molecules, into C. reinhardtii.

摘要

分析了通过电穿孔导入莱茵衣藻的外源DNA的命运。使用单脉冲和双脉冲电刺激，将长达14 kb的质粒导入有完整细胞壁和无完整细胞壁的细胞中。导入后数小时内，外源质粒DNA与从细胞中分离出的细胞核相关联；导入数周后，外源DNA稳定整合到莱茵衣藻基因组中。这些研究确立了电穿孔作为一种将DNA以及潜在的其他分子导入莱茵衣藻的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c2a/359944/f6dd9222ed41/molcellb00138-0567-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c2a/359944/5d963a1748fb/molcellb00138-0566-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c2a/359944/e98afd3e32d2/molcellb00138-0567-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c2a/359944/f6dd9222ed41/molcellb00138-0567-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c2a/359944/5d963a1748fb/molcellb00138-0566-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c2a/359944/e98afd3e32d2/molcellb00138-0567-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c2a/359944/f6dd9222ed41/molcellb00138-0567-b.jpg

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