Błońska Dagmara, Tarkan Ali Serhan, Janic Bartosz, Tszydel Mariusz, Bukowska Bożena
Department of Ecology and Vertebrate Zoology, Faculty of Biology and Environmental Protection University of Lodz Łódź Poland.
Department of Basic Sciences, Faculty of Fisheries Muğla Sıtkı Koçman University Muğla Turkey.
Ecol Evol. 2023 Dec 21;13(12):e10795. doi: 10.1002/ece3.10795. eCollection 2023 Dec.
Changes in oxidative status represent organismal response to stressful external stimuli. While there is substantial knowledge on the influence of abiotic factors on the antioxidant system of different organisms, the impact of biotic factors remains largely unexplored. The aim of the present study was to evaluate the effect of acute competitive interactions on oxidative stress. Territory-resident and intruder round goby individuals were experimentally subjected to competition for limited shelter resource in three treatments (lasting 1, 6 and 12 h), and oxidative stress parameters (total antioxidant capacity, catalase activity, reduced glutathione, lipid peroxidation), as well as behaviour (time spent in the shelter, guarding the shelter and aggression) were measured. All tested biochemical parameters reached higher values in the liver than in the muscle tissue. Fish behaviour and antioxidant defence did not show any potential relationships reflecting changes in antioxidant status and aggression. Particularly, there was no difference between resident and intruder fish in oxidative stress parameters. We compared our results to the outcome of our previous studies (similar experimental protocol and species) but with acute heat shock as a stressor instead of competition. The higher temperature was found to be a stronger stressor than the competition, most pronounced in total antioxidant capacity and oxidative damage.
氧化状态的变化代表了生物体对外部应激刺激的反应。虽然关于非生物因素对不同生物体抗氧化系统的影响已有大量知识，但生物因素的影响在很大程度上仍未得到探索。本研究的目的是评估急性竞争相互作用对氧化应激的影响。将领地居住的和入侵的圆腹雅罗鱼个体在三种处理方式（持续1小时、6小时和12小时）下进行争夺有限庇护资源的实验，并测量氧化应激参数（总抗氧化能力、过氧化氢酶活性、还原型谷胱甘肽、脂质过氧化）以及行为（在庇护所中花费的时间、守护庇护所和攻击行为）。所有测试的生化参数在肝脏中的值均高于肌肉组织。鱼类行为和抗氧化防御未显示出任何反映抗氧化状态和攻击行为变化的潜在关系。特别是，领地居住鱼和入侵鱼在氧化应激参数方面没有差异。我们将我们的结果与之前研究（类似的实验方案和物种）的结果进行了比较，但之前的研究以急性热休克作为应激源而非竞争。结果发现，较高温度是比竞争更强的应激源，在总抗氧化能力和氧化损伤方面最为明显。
