Guan Lingshan, Liu Jinshi, Wang Ruilong, Mu Yanjuan, Sun Tao, Wang Lili, Zhao Yunchao, Zhu Nana, Ji Xinyue, Lu Yizeng, Wang Yan
Key Laboratory of National Forestry and Grassland Administration on Conservation and Utilization of Warm Temperate Zone Forest and Grass Germplasm Resources, Shandong Provincial Center of Forest and Grass Germplasm Resources, Jinan 250102, China.
College of Forestry, Shandong Agricultural University, Tai'an 271018, China.
Biology (Basel). 2023 Nov 25;12(12):1466. doi: 10.3390/biology12121466.
L. is an important landscaping and ornamental tree species throughout southern and northern parts of China. The most common color of petals is yellow and white. In this study, flower color mutants with yellow and white flag petals and light purple-red wing and keel petals were used for transcriptomics and metabolomics analyses. To investigate the underlying mechanisms of flower color variation in 'AM' mutant, 36 anthocyanin metabolites were screened in the anthocyanin-targeting metabolome. The results demonstrated that cyanidins such as cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside in the 'AM' mutant were the key metabolites responsible for the red color of the wing and keel petals. Transcriptome sequencing and differentially expressed gene (DEG) analysis identified the key structural genes and transcription factors related to anthocyanin biosynthesis. Among these, , , , , and expression was significantly correlated with the cyanidin-type anthocyanins (key regulatory factors affecting anthocyanin biosynthesis) in the flag, wing, and keel petals in at various flower development stages.
L.是中国南方和北方重要的园林景观和观赏树种。花瓣最常见的颜色是黄色和白色。在本研究中，以具有黄色和白色旗瓣以及浅紫红色翼瓣和龙骨瓣的花色突变体进行转录组学和代谢组学分析。为了探究‘AM’突变体花色变异的潜在机制，在花青素靶向代谢组中筛选出36种花青素代谢物。结果表明，‘AM’突变体中的花青素如矢车菊素-3-O-葡萄糖苷和矢车菊素-3-O-芸香糖苷是导致翼瓣和龙骨瓣呈现红色的关键代谢物。转录组测序和差异表达基因（DEG）分析确定了与花青素生物合成相关的关键结构基因和转录因子。其中，[此处原文缺失具体基因名称]、[此处原文缺失具体基因名称]、[此处原文缺失具体基因名称]、[此处原文缺失具体基因名称]和[此处原文缺失具体基因名称]的表达与不同花发育阶段L.的旗瓣、翼瓣和龙骨瓣中的矢车菊素型花青素（影响花青素生物合成的关键调控因子）显著相关。
