Wang Ruyi, Yang Xin, Wang Tao, Li Baohui, Li Ping, Zhang Qin
College of Landscape and Tourism, Hebei Agricultural University, Baoding 071000, China.
College of Forestry, Hebei Agricultural University, Baoding 071000, China.
Plants (Basel). 2024 Apr 11;13(8):1077. doi: 10.3390/plants13081077.
Flower color is an important trait that affects the economic value of , a famous ornamental plant in the Rosaceae family. with purple-red flowers is uniquely charming and highly favored in landscape applications. However, little is known about its flower coloring mechanism, which stands as a critical obstacle on the path to innovative breeding for flower color. In this study, transcriptomic and targeted metabolomic analyses of purple-red and white were performed to elucidate the mechanism of flower color formation. In addition, the expression patterns of key genes were analyzed using an RT-qPCR experiment. The results showed that the differential metabolites were significantly enriched in the flavonoid synthesis pathway. A total of 14 anthocyanins emerged as the pivotal metabolites responsible for the differences in flower color between the two cultivars, comprising seven cyanidin derivatives, five pelargonium derivatives, and two paeoniflorin derivatives. Moreover, the results clarified that the metabolic pathway determining flower color in purple-red encompasses two distinct branches: cyanidin and pelargonidin, excluding the delphinidin branch. Additionally, through the integrated analysis of transcriptomic and metabolomic data, we identified 18 key genes responsible for anthocyanin regulation, thereby constructing the gene regulatory network for anthocyanin synthesis. Among them, ten genes (, , , , , , , , , and ) related to anthocyanin synthesis were significantly positively correlated with anthocyanin contents, indicating that they may be the key contributors to anthocyanin accumulation. Our investigation contributes a novel perspective to understanding the mechanisms responsible for flower color formation in . The findings of this study introduce novel strategies for molecular design breeding aimed at manipulating flower color in .
花色是影响[植物名称]经济价值的重要性状，[植物名称]是蔷薇科一种著名的观赏植物。开紫红色花的[植物名称]独具魅力，在园林应用中备受青睐。然而，其花色形成机制却鲜为人知，这成为[植物名称]花色创新育种道路上的关键障碍。在本研究中，对紫红色[植物名称]和白色[植物名称]进行了转录组和靶向代谢组分析，以阐明花色形成机制。此外，通过RT-qPCR实验分析了关键基因的表达模式。结果表明，差异代谢物在类黄酮合成途径中显著富集。共有14种花青素成为导致两个[植物名称]品种花色差异的关键代谢物，包括7种矢车菊素衍生物、5种天竺葵素衍生物和2种芍药苷衍生物。此外，结果表明，决定紫红色[植物名称]花色的代谢途径包括两个不同的分支：矢车菊素和天竺葵素分支，不包括飞燕草素分支。此外，通过对转录组和代谢组数据的综合分析，我们鉴定出18个负责花青素调控的关键基因，从而构建了[植物名称]花青素合成的基因调控网络。其中，10个与花青素合成相关的基因（[基因名称1]、[基因名称2]、[基因名称3]、[基因名称4]、[基因名称5]、[基因名称6]、[基因名称7]、[基因名称8]、[基因名称9]和[基因名称10]）与花青素含量显著正相关，表明它们可能是花青素积累的关键贡献者。我们的研究为理解[植物名称]花色形成机制提供了新的视角。本研究结果为旨在调控[植物名称]花色的分子设计育种引入了新策略。
