不是，是（，）的主要光合共生体。你提供的原文似乎不太完整或存在一些错误，这可能会影响准确理解和翻译的完整性。请检查并补充完整准确的原文以便能更准确地翻译。

, Not , Is the Main Photobiont of (, ).

作者信息

Pino-Bodas Raquel, Blázquez Miguel, de Los Ríos Asunción, Pérez-Ortega Sergio

机构信息

Biodiversity and Conservation Area, Department of Biology and Geology, Physics and Inorganic Chemistry, Rey Juan Carlos University, C/Tulipán s/n, 28933 Móstoles, Spain.

Royal Botanic Gardens, Kew, Richmond, London TW9 3DS, UK.

出版信息

J Fungi (Basel). 2023 Dec 2;9(12):1160. doi: 10.3390/jof9121160.

DOI:10.3390/jof9121160

PMID:38132761

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10744234/

Abstract

This study explores the diversity of photobionts associated with the Mediterranean lichen-forming fungus . For this purpose, we sequenced the whole ITS rDNA region by Sanger using a metabarcoding method for ITS2. A total of 41 specimens from Greece, Italy, France, Portugal, and Spain were studied. Additionally, two specimens from Spain were used to generate four cultures. Our molecular studies showed that the genus is the main photobiont of throughout its geographic distribution. This result contrasts with previous studies, which indicated that the main photobiont for most species is . The identity of was also confirmed by ultrastructural studies of photobionts within the lichen thalli and cultures. Photobiont cells showed a parietal chloroplast lacking a pyrenoid, which characterizes the species in this genus. Phylogenetic analyses indicate hidden diversity within this genus. The results of amplicon sequencing showed the presence of multiple ASVs in 58.3% of the specimens studied.

摘要

本研究探索了与地中海地衣形成真菌相关的共生光合生物的多样性。为此，我们采用针对ITS2的宏条形码方法，通过桑格测序对整个ITS rDNA区域进行了测序。共研究了来自希腊、意大利、法国、葡萄牙和西班牙的41个标本。此外，还使用了来自西班牙的两个标本进行了四次培养。我们的分子研究表明，在其整个地理分布范围内，该属是主要的共生光合生物。这一结果与之前的研究形成了对比，之前的研究表明，大多数该物种的主要共生光合生物是另一种。通过对地衣叶状体和培养物中共生光合生物的超微结构研究，也证实了该属的身份。共生光合生物细胞显示出一个没有蛋白核的周质叶绿体，这是该属物种的特征。系统发育分析表明该属内存在隐藏的多样性。扩增子测序结果显示，在所研究的58.3%的标本中存在多个扩增子序列变体。