Institute of Biology and Ecology, Department of Botany, Safárik University, Mánesova 23, SK-041 67 Kosice, Slovak Republic.
Ecotoxicol Environ Saf. 2010 May;73(4):603-12. doi: 10.1016/j.ecoenv.2009.11.002. Epub 2009 Dec 23.
The photobiont is considered as the more sensitive partner of lichen symbiosis in metal pollution. For this reason the presence of a metal tolerant photobiont in lichens may be a key factor of ecological success of lichens growing on metal polluted substrata. The photobiont inventory was examined for terricolous lichen community growing in Cu mine-spoil heaps derived by historical mining. Sequences of internal transcribed spacer (ITS) were phylogenetically analyzed using maximum likelihood analyses. A total of 50 ITS algal sequences were obtained from 22 selected lichen taxa collected at three Cu mine-spoil heaps and two control localities. Algae associated with Cladonia and Stereocaulon were identified as members of several Asterochloris lineages, photobionts of cetrarioid lichens clustered with Trebouxia hypogymniae ined. We did not find close relationship between heavy metal content (in localities as well as lichen thalli) and photobiont diversity. Presence of multiple algal genotypes in single lichen thallus has been confirmed.
在金属污染中，共生藻被认为是地衣共生体中更敏感的伙伴。出于这个原因，在生长在金属污染基质上的地衣中存在耐受金属的共生藻可能是地衣生态成功的关键因素。对在由历史采矿产生的铜矿废石堆上生长的陆生地衣群落进行了共生藻目录检查。使用最大似然分析对内部转录间隔区（ITS）的序列进行了系统发育分析。从在三个铜矿废石堆和两个对照地点采集的 22 种地衣分类群中获得了总共 50 个 ITS 藻类序列。与 Cladonia 和 Stereocaulon 相关的藻类被鉴定为几个 Asterochloris 谱系的成员，与 Cetrarioid 地衣相关的共生藻与 Trebouxia hypogymniae ined 聚类。我们没有发现重金属含量（在地点和地衣体中）与共生藻多样性之间存在密切关系。已经证实单个地衣体中存在多种藻类基因型。
