临床实验室中嗜麦芽窄食单胞菌的鉴定。
Identification of Pseudomonas pseudomallei in the clinical laboratory.
作者信息
Ashdown L R
出版信息
J Clin Pathol. 1979 May;32(5):500-4. doi: 10.1136/jcp.32.5.500.
Abstract
Ninety-one strains of Pseudomonas pseudomallei were tested in the API 20E system and in equivalent conventional tests. Except for utilisation of citrate there was good correlation between API and conventional tests. Seven-digit profiles were constructed from each strain after 48 hours' incubation, and numerical codes 2 006 727, 2 206 706, 2 206 707, and 2 206 727 accounted for 77% of strains tested. API 20 E, in combination with tests for heat stability of alkaline phosphatase, resistance to colistin and gentamicin, oxidative attack only of glucose, and acid from maltose, was found to provide a simple method for positive identification of all strains of this organism in two days.
摘要
对91株类鼻疽假单胞菌进行了API 20E系统测试及等效的传统测试。除柠檬酸盐利用情况外，API测试与传统测试之间具有良好的相关性。孵育48小时后，为每个菌株构建了七位数的谱型，数字编码2 006 727、2 206 706、2 206 707和2 206 727占测试菌株的77%。结果发现，API 20 E与碱性磷酸酶热稳定性测试、对黏菌素和庆大霉素的耐药性测试、仅对葡萄糖的氧化攻击测试以及麦芽糖产酸测试相结合，可提供一种在两天内对该生物体所有菌株进行阳性鉴定的简单方法。
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