Oberhofer T R
J Clin Microbiol. 1979 Feb;9(2):220-6. doi: 10.1128/jcm.9.2.220-226.1979.
The API 20E and Oxi/Ferm systems were tested in parallel to identify nonfermentative bacteria and oxidase-positive fermentative bacteria. Test strains consisted of consecutive clinical isolates, with stock cultures used to supplement those species infrequently recovered. The two microsystems, as well as tubes of triple sugar iron, motility, cetrimide, and oxidative glucose media, were inoculated by each worker for each organism. Identification of each isolate was by the protocol of the manufacturers, with supplemental tests and flagella stains performed when necessary. Concurrent identification was undertaken with a conventional system against which the results of the two systems were compared for accuracy. There was a 95.3% accuracy in identification by the Oxi-Ferm system and 88.9% by the API system. Almost one-fourth of all identification attempts with the API required computer assistance, and most of these were for oxidase positive bacteria. Because of this, and because the API system showed greater accuracy in identification of the oxidase-negative bacteria, it seems best suited for identification of these organisms (P. maltophilia, A. anitratus, and A. lwoffi). The Oxi/Ferm system is technically less cumbersome than the API and is well suited for both groups of organisms.
同时对API 20E系统和Oxi/Ferm系统进行测试，以鉴定非发酵菌和氧化酶阳性发酵菌。测试菌株包括连续的临床分离株，并用储备培养物补充那些不常分离到的菌种。每个工作人员针对每种微生物接种这两种微量系统以及三糖铁、动力、溴化十六烷基三甲铵和氧化葡萄糖培养基试管。每种分离株均按照制造商的方案进行鉴定，必要时进行补充试验和鞭毛染色。使用传统系统进行同步鉴定，并将这两种系统的结果与之比较以确定准确性。Oxi-Ferm系统的鉴定准确率为95.3%，API系统为88.9%。使用API进行的所有鉴定尝试中，近四分之一需要计算机辅助，其中大多数是针对氧化酶阳性细菌。因此，鉴于API系统在氧化酶阴性细菌的鉴定中显示出更高的准确性，它似乎最适合鉴定这些微生物（嗜麦芽窄食单胞菌、产碱杆菌和洛菲不动杆菌）。Oxi/Ferm系统在技术上比API系统更简便，适用于这两类微生物。
