Alonso Cristina, Martí Meritxell, Ramos Anderson, Calpena Ana Cristina, Clares-Naveros Beatriz, Coderch Luisa
Surfactants and Nanobiotechnology Department, Institute of Advanced Chemical of Catalonia of CSIC, (IQAC-CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain.
Department of Pharmacy and Pharmaceutical Technology, Universitat de Barcelona, Avda. Joan XXIII s/n, 08028 Barcelona, Spain.
Membranes (Basel). 2023 Dec 12;13(12):905. doi: 10.3390/membranes13120905.
The main objective of this study is the evaluation of the use of a synthetic membrane, Nuclepore, as a model for permeation studies through sublingual mucosa. The permeability of two types of membranes, porcine sublingual oral mucosa and a synthetic Nuclepore membrane, to water was compared. Moreover, the water permeability of membranes modified with waterproofing formulations was measured to study their ability to protect against the penetration of viruses, toxins, etc. A relatively high correlation (R 0.88) was obtained between the transmucosal water loss (TMWL) values of the artificial membrane and the mucosa. These results support the possible use of this synthetic membrane in the screening of the water permeability of formulations. In addition, studies of the permeation of different actives, drugs, and biocides through the two membranes were carried out, and these results were compared with their skin permeation data. The synthetic membrane does not seem to discern between compounds in terms of permeability. However, the permeation of caffeine through intact or modified membranes incorporating waterproofing formulations presents similar permeation profiles through the synthetic membrane and mucosa. The results from these assays should lend support to the use of this synthetic membrane when screening formulations to be applied in oral penetration studies.
本研究的主要目的是评估使用合成膜Nuclepore作为透过舌下黏膜渗透研究模型的情况。比较了两种类型的膜，即猪舌下口腔黏膜和合成Nuclepore膜对水的渗透性。此外，还测量了用防水制剂改性的膜的水渗透性，以研究它们防止病毒、毒素等渗透的能力。人工膜和黏膜的经黏膜水分流失（TMWL）值之间获得了相对较高的相关性（R = 0.88）。这些结果支持了这种合成膜在筛选制剂水渗透性方面的可能用途。此外，还进行了不同活性成分、药物和杀菌剂透过这两种膜的渗透研究，并将这些结果与其皮肤渗透数据进行了比较。合成膜在渗透性方面似乎无法区分化合物。然而，咖啡因透过含有防水制剂的完整或改性膜在合成膜和黏膜上呈现出相似的渗透曲线。这些试验结果应有助于支持在筛选用于口腔渗透研究的制剂时使用这种合成膜。
