College of Chemistry, Changchun Normal University, Changchun 130032, China.
College of Chemistry, Changchun Normal University, Changchun 130032, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Mar 5;308:123798. doi: 10.1016/j.saa.2023.123798. Epub 2023 Dec 19.
A highly sensitive surface-enhanced Raman spectroscopy approach was established for the detection of dextromethorphan hydrobromide (DXM) utilizing nano CuO modified silver nanoparticles (CuO@AgNPs) as substrate. Ultraviolet visible spectra (UV-vis), X-ray diffraction (XRD) and transmission electron microscopy (TEM) characterized the synthesized CuO@AgNPs. UV-vis and fourier transform infrared (FT-IR) were adopted to investigate the interaction between DXM and CuO@AgNPs. The optimal experimental conditions (the dosages of CuO@AgNPs and NaCl as well as mixing time) were explored. The enhancement factor (EF) was 1.71 × 10. The linear relationship between SERS intensity and the concentration of DXM in the range of 67 - 1000 nmol L was obtained as I = 25.81 c + 40398.77, and the limit of detection (LOD) was 2.12 nmol L (S/N = 3). The interference of K, Mg, Zn, Ca, Cu, Fe, glucose, HSA, L-tryptophan, soluble starch and ibuprofen were investigated. The method was successfully applied to test DXM in serum samples. The recovery was 99.06% - 101.51% with the relative standard deviation (RSD) of 0.74% - 3.87%.
建立了一种基于纳米氧化铜修饰的银纳米粒子（CuO@AgNPs）作为基底的高灵敏度表面增强拉曼光谱法，用于检测氢溴酸右美沙芬（DXM）。采用紫外可见光谱（UV-vis）、X 射线衍射（XRD）和透射电子显微镜（TEM）对合成的 CuO@AgNPs 进行了表征。采用紫外可见光谱和傅里叶变换红外光谱（FT-IR）研究了 DXM 与 CuO@AgNPs 之间的相互作用。考察了最佳实验条件（CuO@AgNPs 和 NaCl 的用量以及混合时间）。得到了 SERS 强度与 DXM 浓度在 67-1000 nmol L 范围内的线性关系，I = 25.81 c + 40398.77，检测限（LOD）为 2.12 nmol L（S/N = 3）。考察了 K、Mg、Zn、Ca、Cu、Fe、葡萄糖、HSA、L-色氨酸、可溶性淀粉和布洛芬的干扰。该方法成功应用于血清样品中 DXM 的检测。回收率为 99.06%-101.51%，相对标准偏差（RSD）为 0.74%-3.87%。
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