College of Chemistry, Changchun Normal University, Changchun 130032, China.
College of Chemistry, Changchun Normal University, Changchun 130032, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Jan 15;285:121942. doi: 10.1016/j.saa.2022.121942. Epub 2022 Oct 5.
A surface-enhanced Raman spectroscopy (SERS) method for the determination of isepamicin (ISE) using silver nanoparticles (AgNPs) protected by bovine serum albumin (BSA) and modified by α-FeO as an efficient substrate was established. The synthesized substrate was characterized and verified by transmission electron microscopy (TEM), ultraviolet visible spectroscopy (UV-vis), and fourier transform infrared spectroscopy (FT-IR). The conditions affecting the Raman signal of ISE were optimized by single factor and response surface experiments. Under optimized conditions, a standard curve I = 43.08c + 63598.69 (c: nmol/L) with a linear relationship (r = 0.9976) was established between the SERS intensity and ISE concentration in the range of 20.00 - 2000.00 nmol/L. The limit of detection (LOD) for ISE was 16.58 nmol/L (S/N = 3). The recovery of ISE in the samples was 96.29 % - 104.12 %, with relative standard deviation (RSD) was 1.53 % - 3.43 % (n = 5). The SERS method was reliable and satisfactory for the quantitative analysis of ISE at low concentration.
建立了一种使用牛血清白蛋白（BSA）保护的银纳米粒子（AgNPs）和α-FeO 修饰的银纳米粒子作为高效基底的表面增强拉曼光谱（SERS）法来测定异帕米星（ISE）。通过透射电子显微镜（TEM）、紫外可见光谱（UV-vis）和傅里叶变换红外光谱（FT-IR）对合成的基底进行了表征和验证。通过单因素和响应面实验优化了影响 ISE 拉曼信号的条件。在优化条件下，在 20.00 - 2000.00 nmol/L 范围内，ISE 的 SERS 强度与 ISE 浓度之间建立了标准曲线 I = 43.08c + 63598.69（c：nmol/L），线性关系良好（r = 0.9976）。ISE 的检出限（LOD）为 16.58 nmol/L（S/N = 3）。ISE 在样品中的回收率为 96.29% - 104.12%，相对标准偏差（RSD）为 1.53% - 3.43%（n = 5）。该 SERS 方法可靠且令人满意，可用于低浓度 ISE 的定量分析。
