College of Chemistry, Changchun Normal University, Changchun 130032, China.
College of Chemistry, Changchun Normal University, Changchun 130032, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Feb 5;326:125181. doi: 10.1016/j.saa.2024.125181. Epub 2024 Sep 21.
An efficient Surface-enhanced Raman scattering (SERS) method for the detection of cysteamine hydrochloride (CSH) was developed by synthesizing a composite substrate comprising silver nanoparticles (AgNPs) functionalized with MoS and β-cyclodextrin (β-CD). The enhanced Raman signals of CSH by β-CD/MoS/AgNPs substrate were the contribution of electromagnetic enhancement (EM) as well as chemical enhancement (CM), and the enhancement factor (EF) can reach up to 3.11 × 10 (peak at 633 cm). Various instrumental techniques were used to characterize the substrate, such as X-ray diffraction (XRD), thermogravimetric analysis (TGA), transmission electron microscopy (TEM), high-angle annular dark field scanning transmission electron microscopy (HAADF-STEM) and ultraviolet visible (UV-vis). The binding of β-CD/MoS/AgNPs and CSH was confirmed by UV-vis and Fourier transform infrared (FT-IR). The optimal experimental conditions were determined by single factor experiments as well as response surface model. The influences of different metal ions and analogous drugs on the detection of CSH were investigated. Under optimum conditions, a good linear correlation (R = 0.9997) was established for CSH in the range of 10.00-1000.00 nmol/L, and the limit of detection (LOD) was as low as 0.78 nmol/L (S/N = 3). The contents of CSH in meat samples were detected. The recovery was 96.6-103.1 %, and the relative standard deviation (RSD) of the measurement was 0.7-3.9 % (n = 7).
一种高效的表面增强拉曼散射（SERS）方法，用于检测半胱胺盐酸盐（CSH），通过合成一种包含银纳米粒子（AgNPs）的复合材料基底来实现，该基底功能化有 MoS 和 β-环糊精（β-CD）。β-CD/MoS/AgNPs 基底对半胱胺盐酸盐（CSH）的增强拉曼信号是电磁增强（EM）和化学增强（CM）的贡献，增强因子（EF）可达 3.11×10（在 633cm 处的峰值）。使用各种仪器技术对基底进行了表征，例如 X 射线衍射（XRD）、热重分析（TGA）、透射电子显微镜（TEM）、高角度环形暗场扫描透射电子显微镜（HAADF-STEM）和紫外可见（UV-vis）。通过紫外可见和傅里叶变换红外（FT-IR）证实了β-CD/MoS/AgNPs 和 CSH 的结合。通过单因素实验和响应面模型确定了最佳实验条件。考察了不同金属离子和类似药物对 CSH 检测的影响。在最佳条件下，CSH 在 10.00-1000.00nmol/L 范围内建立了良好的线性关系（R=0.9997），检测限（LOD）低至 0.78nmol/L（S/N=3）。检测了肉样中的 CSH 含量。回收率为 96.6-103.1%，测量的相对标准偏差（RSD）为 0.7-3.9%（n=7）。
