Zhang Yujiao, Song Haoran, Liu Zhengqi, Ai Chunqing, Yan Chunhong, Dong Xiuping, Song Shuang
Liaoning Key Laboratory of Food Nutrition and Health, Collaborative Innovation Center of Seafood Deep Processing, National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
Foods. 2023 Dec 14;12(24):4476. doi: 10.3390/foods12244476.
Due to its significant physiological effects, a sulfated polysaccharide has been considered an important nutrient of sea cucumber, but its metabolism in vivo is still unclear. The present study investigated the metabolism of a sea cucumber sulfated polysaccharide (SCSP) in rats and its influence on the metabolite profiles. The quantification by HPLC-MS/MS revealed that the blood level of SCSP achieved a maximum of 54.0 ± 4.8 μg/mL at 2 h after gavage, almost no SCSP was excreted through urine, and 55.4 ± 29.8% of SCSP was eliminated through feces within 24 h. These results prove the utilization of SCSP by gut microbiota, and a further microbiota sequencing analysis indicated that the SCSP utilization in the gut was positively correlated with Muribaculaceae and Clostridia_UCG-014. In addition, the non-targeted metabolomic analysis demonstrated the significant effects of SCSP administration on the metabolite profiles of blood, urine, and feces. It is worth noting that the SCSP supplement decreased palmitic acid, stearic acid, and oleic acid in blood and urine while increasing stearic acid, linoleic acid, and γ-linolenic acid in feces, suggesting the inhibition of fat absorption and the enhancement of fat excretion by SCSP, respectively. The present study shed light on the metabolism in vivo and the influence on the fat metabolism of SCSP.
由于其显著的生理作用，硫酸化多糖被认为是海参的一种重要营养成分，但其在体内的代谢仍不清楚。本研究调查了海参硫酸化多糖（SCSP）在大鼠体内的代谢及其对代谢物谱的影响。通过HPLC-MS/MS定量分析发现，灌胃后2小时SCSP的血药浓度达到最大值54.0±4.8μg/mL，几乎没有SCSP通过尿液排泄，并且在24小时内55.4±29.8%的SCSP通过粪便排出。这些结果证明了肠道微生物群对SCSP的利用，进一步的微生物群测序分析表明，肠道中SCSP的利用与Muribaculaceae和Clostridia_UCG-014呈正相关。此外，非靶向代谢组学分析表明，给予SCSP对血液、尿液和粪便的代谢物谱有显著影响。值得注意的是，补充SCSP可降低血液和尿液中的棕榈酸、硬脂酸和油酸，同时增加粪便中的硬脂酸、亚油酸和γ-亚麻酸，分别表明SCSP对脂肪吸收的抑制和对脂肪排泄的促进作用。本研究揭示了SCSP在体内的代谢及其对脂肪代谢的影响。
