Danilova Natalia, Galieva Gulnaz, Kuryntseva Polina, Selivanovskaya Svetlana, Galitskaya Polina
Institute of Environmental Sciences, Kazan Federal University, Kazan 420008, Russia.
Microorganisms. 2023 Nov 21;11(12):2828. doi: 10.3390/microorganisms11122828.
Antibiotics enter the soil with compost prepared from livestock manures and other sources. There is concern that they may influence plant growth and cause antibiotic resistance in soil and plant endospheric microbiomes. In the present work, lettuce plants were cultivated in soil and hydroponics spiked with oxytetracycline (0, 15, and 300 mg × kg and 0, 15, and 50 mg × L, respectively) during a 28-day greenhouse experiment. It was revealed that the antibiotic reduced the chlorophyll content, the biomass, and the length of the roots and stems by 1.4-4.7, 1.8-39, 2.5-3.2, and 1.8-6.3 times in soil and in hydroponics. The copy numbers of the (A) and (X) genes were revealed to be 4.51 × 10-1.58 × 10 and 8.36 × 10-1.07 × 10 copies × g, respectively, suggesting the potential migration of these genes from soil/hydroponics to plant roots and leaves. According to a non-metric multidimensional scaling (NMDS) analysis of the 16S rRNA amplicon sequencing data, endospheric bacterial communities were similar in leaves and roots independent of the growing substrate and antibiotic concentration. While soil bacterial communities were unaffected by the presence of antibiotics, hydroponic communities exhibited dependency, likely attributable to the absence of the mitigating effect of soil particle absorption.
抗生素通过由牲畜粪便和其他来源制备的堆肥进入土壤。人们担心它们可能会影响植物生长，并在土壤和植物内生微生物群落中产生抗生素抗性。在本研究中，在为期28天的温室实验期间，将生菜种植在添加了土霉素（分别为0、15和300 mg/kg以及0、15和50 mg/L）的土壤和水培环境中。结果表明，抗生素使土壤和水培环境中的叶绿素含量、生物量以及根和茎的长度分别降低了1.4 - 4.7倍、1.8 - 39倍、2.5 - 3.2倍和1.8 - 6.3倍。(A)和(X)基因的拷贝数分别为4.51×10 - 1.58×10和8.36×10 - 1.07×10拷贝/g，表明这些基因可能从土壤/水培环境迁移到植物根和叶中。根据对16S rRNA扩增子测序数据的非度量多维尺度分析（NMDS），无论生长基质和抗生素浓度如何，内生细菌群落在叶片和根部相似。虽然土壤细菌群落不受抗生素存在的影响，但水培群落表现出依赖性，这可能归因于土壤颗粒吸附的缓解作用不存在。
