生肌化瘀方醇提物部位对链脲佐菌素诱导糖尿病大鼠溃疡创面愈合作用的研究。
Research on the wound healing effect of Shengji Huayu Formula ethanol extract-derived fractions in streptozotocin-induced diabetic ulcer rats.
机构信息
Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200437, China.
Department of Pharmacy, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Yueyang, 200437, China.
出版信息
BMC Complement Med Ther. 2023 Mar 1;23(1):67. doi: 10.1186/s12906-023-03894-0.
BACKGROUND
Diabetic ulcer is a common complication of diabetes. It is characterized by a long-term disease course and high recurrence rate. Shengji Huayu Formula (SHF) is an effective formula for treating diabetic ulcers. However, the specific effective parts of SHF remain unclear. Clarifying the active polar site of SHF would be helpful to refine research on the components in SHF that promote wound healing. This research aims to focus on evaluating the activity of polar fractions.
METHODS
A diabetic rat model was established by intraperitoneally injecting streptozotocin (STZ) and was adopted to confirm the therapeutic effect of SHF. Four different polarity parts were extracted from SHF and prepared into a cream to evaluate the activity. High-performance liquid chromatography (HPLC) was used to detect chemical constituents in chloroform extracts.
RESULTS
It was discovered that dracorhodin, aloe-emodin, rhein, imperatorin, emodin, isoimperatorin, chrysophanol, physcion, and tanshinone IIA were the main components of the chloroform extract from SHF. The results revealed that chloroform extract could effectively accelerate diabetic wound healing by promoting collagen regeneration and epidermal repair. Chloroform extract of SHF could stimulate the generation of vascular endothelial growth factor (VEGF). The results are also indicated that the effective active fraction was the chloroform part, and the method of detecting the main chemical constituents in the active part was successfully established.
CONCLUSION
SHF could improve diabetic ulcers by promoting granulation tissue synthesis. In this study, four polar parts (petroleum ether, chloroform, ethylacetate, n-butanol) were extracted from a 95% ethanol extract. In contrast, chloroform polar parts showed a higher wound closure rate, stimulated more collagen regeneration and promoted more production of vascular endothelial cells. In conclusion, the chloroform extract of SHF was the effective polar part in ameliorating diabetic wound healing.
背景
糖尿病溃疡是糖尿病的常见并发症。其具有病程长、复发率高的特点。生肌化瘀方（SHF）是治疗糖尿病溃疡的有效方剂。然而，SHF 的具体有效部位尚不清楚。明确 SHF 的活性极性部位有助于深入研究促进伤口愈合的 SHF 成分。本研究旨在重点评估极性馏分的活性。
方法
通过腹腔注射链脲佐菌素（STZ）建立糖尿病大鼠模型，验证 SHF 的治疗效果。从 SHF 中提取四种不同极性部分并制成乳膏，以评估其活性。采用高效液相色谱法（HPLC）检测氯仿提取物中的化学成分。
结果
发现从 SHF 的氯仿提取物中主要有血根碱、大黄素、大黄酸、大茴香脑、大黄素、异大黄素、大黄酚、大黄素甲醚、丹参酮ⅡA 等成分。结果表明，氯仿提取物可通过促进胶原再生和表皮修复有效加速糖尿病创面愈合。SHF 氯仿提取物可刺激血管内皮生长因子（VEGF）的产生。结果还表明，有效活性部位是氯仿部分，成功建立了检测活性部位主要化学成分的方法。
结论
SHF 可通过促进肉芽组织合成来改善糖尿病溃疡。本研究从 95%乙醇提取物中提取了四个极性部分（石油醚、氯仿、乙酸乙酯、正丁醇），相比之下，氯仿极性部分的创面闭合率更高，刺激胶原再生更多，促进血管内皮细胞产生更多。综上所述，SHF 的氯仿提取物是改善糖尿病创面愈合的有效极性部位。
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