College of Agronomy, Ningxia University, Yinchuan 750021, China.
Ningxia Research Institute of Quality Standards and Testing Technology of Agricultural Products, Yinchuan 750001, China.
Molecules. 2023 Dec 14;28(24):8088. doi: 10.3390/molecules28248088.
As a beneficial nutrient and essential trace element, selenium plays a significant role in plant growth functions and human protein biosynthesis. Plant selenium enrichment is mainly obtained from both natural soil and exogenous selenium supplementation, while human beings consume selenium-enriched foods for the purposes of selenium supplementation. In this study, different types of selenium fertilizers were sprayed onto in Ningxia, and transcriptomics and metabolomics techniques were used to explore the effects of selenium on the fruit differentials and differential genes in . Taking the "Ning Qiyi No.1" wolfberry as the research object, sodium selenite, nano-selenium, and organic selenium were sprayed at a concentration of 100 mg·L three times from the first fruiting period to the harvesting period, with a control treatment comprising the spraying of clear water. We determined the major metabolites and differential genes of the amino acids and derivatives, flavonoids, and alkaloids in ripe wolfberries. We found that spraying selenium significantly enhanced the metabolic differentiators; the most effective spray was the organic selenium, with 129 major metabolic differentiators and 10 common metabolic pathways screened after spraying. Nano-selenium was the next best fertilizer we screened, with 111 major metabolic differentiators, the same number as organic selenium in terms of differential genes and common metabolite pathways. Sodium selenite was the least effective of the three, with only 59 of its major metabolic differentials screened, but its differential genes and metabolites were enriched for five common pathways.
作为一种有益的营养物质和必需微量元素，硒在植物生长功能和人类蛋白质生物合成中发挥着重要作用。植物富硒主要来源于自然土壤和外源硒的补充，而人类则通过食用富硒食品来补充硒。本研究在宁夏喷施不同类型硒肥，采用转录组学和代谢组学技术，探讨硒对枸杞果实差异及差异基因的影响。以“宁杞 1 号”枸杞为研究对象，在果实始期、盛期、末期喷施 100mg·L-1 的亚硒酸钠、纳米硒、有机硒 3 次，清水对照。测定了成熟枸杞中氨基酸及衍生物、类黄酮、生物碱的主要代谢物和差异基因。结果表明，喷施硒能显著提高枸杞的代谢物差异；其中有机硒效果最好，喷施后筛选出 129 个主要代谢物差异和 10 条共同代谢途径。纳米硒是我们筛选出的第二种效果较好的肥料，有 111 个主要代谢物差异，在差异基因和共同代谢途径方面与有机硒相同。亚硒酸钠是三种中效果最差的，仅筛选出 59 个主要代谢差异，但差异基因和代谢产物富集了 5 条共同途径。
