Yang Meng-Ke, Liu Sai, Li Jian-Ling, Guo Kun, Qiao Hai-Li, Xu Rong, Xu Chang-Qing, Chen Jun
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193, China.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193, China China Resources Sanjiu Medical & Pharmaceutical Co., Ltd. Shenzhen 518110, China.
Zhongguo Zhong Yao Za Zhi. 2020 Jun;45(12):2842-2846. doi: 10.19540/j.cnki.cjcmm.20200329.109.
Aceria pallida is one of the most common pests in the main production areas of Lycium barbarum in China. The mite mainly feeds on foliage, leading to local tissue deformation and formation of massive galls, which seriously affects the growth and yield of L. barbarum. However, little is known about the influence of galling organisms on plant primary and secondary metabolism. In order to compare the metabolites differences between healthy and the mite infested leaves of wolfberry, and provide a scientific basis for the development and utilization of the galled leaves, L. barbarum seedlings were infested with A. pallida artificially in the laboratory, the metabolites of L. barbarum leaves were determined by LC-MS/MS. Our results showed that the leaves were rich in amino acids and flavonoid compounds. A total of 204 compounds from 16 classes were detected in L. barbarum leaves based on LC-MS/MS. The primary metabolites are mainly amino acids, and the secondary metabolites are mainly organic acids and flavonoids. The content of the metabolite in the leaves of L. barbarum was significantly affected by the mite, 30 metabolites such as flavonoids and phenylpropanoids were significantly changed, 21 metabolites were up-regulated and 9 metabolites were down-regulated significantly. There were 8 compounds which has pharmacological and biological activity, such as eriodictyol, isorhamnetin-3-O-neohesperidoside and scopoletin up-regulated significantly. Based on the above findings, we suggest that the galled leaves of L.barbarum have a potential to be developed in the future.
枸杞瘿螨是我国枸杞主要产区最常见的害虫之一。该螨主要取食叶片，导致局部组织变形并形成大量虫瘿，严重影响枸杞的生长和产量。然而，关于致瘿生物对植物初级和次级代谢的影响知之甚少。为了比较枸杞健康叶片和受螨侵害叶片的代谢物差异，并为虫瘿叶的开发利用提供科学依据，在实验室中人工用枸杞瘿螨侵染枸杞幼苗，采用液相色谱-串联质谱法（LC-MS/MS）测定枸杞叶片的代谢物。我们的结果表明，叶片富含氨基酸和黄酮类化合物。基于LC-MS/MS在枸杞叶片中总共检测到16类204种化合物。初级代谢物主要是氨基酸，次级代谢物主要是有机酸和黄酮类化合物。枸杞瘿螨显著影响枸杞叶片中代谢物的含量，30种代谢物如黄酮类和苯丙烷类显著变化，21种代谢物上调，9种代谢物显著下调。有8种具有药理和生物活性的化合物，如圣草酚、异鼠李素-3-O-新橙皮糖苷和东莨菪素显著上调。基于上述发现，我们认为枸杞虫瘿叶未来有开发潜力。
