Wolfberry Engineering Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, National Wolfberry Engineering Research Center, Yinchuan 750002, China.
College of Horticulture, Northwest A & F University, Yangling, Xianyang 712000, China.
Molecules. 2019 Oct 28;24(21):3879. doi: 10.3390/molecules24213879.
The yield and quality of goji ( L.) fruit are heavily dependent on fertilizer, especially the availability of nitrogen, phosphorus, and potassium (N, P, and K, respectively). In this study, we performed a metabolomic analysis of the response of goji berry to nitrogen fertilizer levels using an Ultra Performance Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry (UPLC-ESI-MS/MS) method. There was no significant difference in the fruit yield or the commodity grade between N0 (42.5 g/plant), N1 (85 g/plant), and N2 (127.5 g/plant). The primary nutrients of the goji berry changed with an increasing nitrogen fertilization. Comparative metabolomic profiling of three nitrogen levels resulted in the identification of 612 metabolites, including amino acids, flavonoids, carbohydrates, organic acids, and lipids/alcohols, among others, of which 53 metabolites (lipids, fatty acids, organic acids, and phenolamides) demonstrated significant changes. These results provide new insights into the molecular mechanisms of the relationship between yield and quality of goji berry and nitrogen fertilizer.
枸杞（L.）果实的产量和品质严重依赖于肥料，特别是氮（N）、磷（P）和钾（K）的有效性。在这项研究中，我们使用超高效液相色谱-电喷雾电离-串联质谱（UPLC-ESI-MS/MS）方法对枸杞果实对氮肥水平的响应进行了代谢组学分析。N0（42.5 g/株）、N1（85 g/株）和 N2（127.5 g/株）三个氮水平处理之间的果实产量和商品等级没有显著差异。枸杞果实的主要营养成分随氮施肥量的增加而变化。对三个氮水平的比较代谢组学分析鉴定出 612 种代谢物，包括氨基酸、类黄酮、碳水化合物、有机酸和脂类/醇类等，其中 53 种代谢物（脂类、脂肪酸、有机酸和酚酰胺）表现出显著变化。这些结果为枸杞产量和品质与氮肥关系的分子机制提供了新的见解。
