Jadranin Milka, Savić Danica, Lupšić Ema, Podolski-Renić Ana, Pešić Milica, Tešević Vele, Milosavljević Slobodan, Krstić Gordana
University of Belgrade-Institute of Chemistry, Technology and Metallurgy, Department of Chemistry, Njegoševa 12, 11000 Belgrade, Serbia.
Department of Neurobiology, Institute for Biological Research "Siniša Stanković"-National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, Serbia.
Plants (Basel). 2023 Dec 16;12(24):4181. doi: 10.3390/plants12244181.
ssp. Necker (ES) and (EC) with a habitat in the Deliblato Sands were the subject of this examination. The latexes of these so far insufficiently investigated species of the genus are used in traditional medicine for the treatment of wounds and warts on the skin. To determine their chemical composition, non-targeted screening of the latexes' chloroform extracts was performed using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry employing an electrospray ionization source (LC-ESI QTOF MS). The analysis of the obtained results showed that the latexes of ES and EC represent rich sources of diterpenes, tentatively identified as jatrophanes, ingenanes, tiglianes, myrsinanes, premyrsinanes, and others. Examination of the anticancer activity of the ES and EC latex extracts showed that both extracts significantly inhibited the growth of the non-small cell lung carcinoma NCI-H460 and glioblastoma U87 cell lines as well as of their corresponding multi-drug resistant (MDR) cell lines, NCI-H460/R and U87-TxR. The obtained results also revealed that the ES and EC extracts inhibited the function of P-glycoprotein (P-gp) in MDR cancer cells, whose overexpression is one of the main mechanisms underlying MDR.
本次研究对象为分布于德利布拉托沙丘（Deliblato Sands）的Necker（ES）和（EC）亚种。该属目前研究尚不充分的这些物种的乳胶在传统医学中用于治疗皮肤伤口和疣。为确定其化学成分，采用电喷雾电离源的液相色谱-四极杆飞行时间质谱联用仪（LC-ESI QTOF MS）对乳胶的氯仿提取物进行了非靶向筛选。对所得结果的分析表明，ES和EC的乳胶富含二萜类化合物，初步鉴定为麻风树烷型、瑞香烷型、大戟烷型、紫金牛烷型、前紫金牛烷型等。对ES和EC乳胶提取物的抗癌活性检测表明，两种提取物均能显著抑制非小细胞肺癌NCI-H460和胶质母细胞瘤U87细胞系及其相应的多药耐药（MDR）细胞系NCI-H460/R和U87-TxR的生长。所得结果还表明，ES和EC提取物可抑制MDR癌细胞中P-糖蛋白（P-gp）的功能，其过表达是MDR的主要机制之一。
