Kato Y, Hirachi Y, Toda Y, Takemasa N, Kotani S
Biken J. 1986 Jun;29(2):39-44.
The experimental conditions under which protoplasts of Staphylococcus aureus strain MS353 (pCp) are converted to the coccal or L-form were investigated. Protoplasts prepared by treating coccal MS353 (pCp) strain with Lysostaphin formed various types of colonies (coccal form, L-form and mixed types) in about 50% yield when they were plated on reversion (R) medium consisting of 2% brain heart infusion, 0.5M sodium succinate, 0.01% bovine serum albumin, 20 mM MgCl2 and 0.6% agar. The L-form type colonies with a typical fried-egg appearance that developed on the R medium at an early stage gradually reverted to the coccal form through a mixed type stage in which a high density area first appeared in the periphery of the colony and then spread throughout the colony. The use of modified R medium without MgCl2 or R medium in which 0.5M sodium succinate as an osmotic stabilizer was replaced by 7.5% NaCl resulted in marked delay in the appearance of reverted cells. R medium without bovine serum albumin yielded atypical L-form type colonies, which contained masses of coccal cells with very irregular margins. On the other hand, R medium without MgCl2 but with penicillin G supported development of L-form type colonies at high rate (13-15%) from the inoculated protoplasts.
研究了金黄色葡萄球菌MS353（pCp）菌株原生质体转化为球菌或L型的实验条件。用溶葡萄球菌素处理球菌MS353（pCp）菌株制备的原生质体，当接种在由2%脑心浸液、0.5M琥珀酸钠、0.01%牛血清白蛋白、20mM氯化镁和0.6%琼脂组成的回复（R）培养基上时，约50%的原生质体形成了各种类型的菌落（球菌型、L型和混合型）。在R培养基上早期形成的具有典型煎蛋外观的L型菌落，通过一个混合类型阶段逐渐回复为球菌型，在这个阶段，菌落周边首先出现高密度区域，然后扩散到整个菌落。使用不含氯化镁的改良R培养基或用7.5%氯化钠替代0.5M琥珀酸钠作为渗透稳定剂的R培养基，会导致回复细胞出现明显延迟。不含牛血清白蛋白的R培养基产生非典型的L型菌落，其中含有大量边缘非常不规则的球菌细胞。另一方面，不含氯化镁但含有青霉素G的R培养基，接种的原生质体可高频率（13 - 15%）形成L型菌落。
