Ikeda M
Department of Microbiology, Faculty of Dentistry, Kyushu University, Fukuoka.
Fukuoka Igaku Zasshi. 1993 Sep;84(9):402-10.
The L-form growth of Staphylococcus aureus induced by ampicillin or fosfomycin was found to be severely depressed by horse or bovine serum included in the culture medium. Fractionation of horse serum revealed that the inhibitory activity was copurified with high-density lipoprotein through floating ultracentrifugation, gel filtration, and isoelectric focusing. It was also shown that the inhibition was reversible: ampicillin-induced L-form cells lost their ability to form L-form colonies when mixed with the lipoprotein fraction or serum before plating, but the colony-forming ability was restored to the original level by treatment of the mixture with a protease, while simple dilution was ineffective. A mechanism for the growth inhibition remains to be clarified.
发现培养基中所含的马血清或牛血清会严重抑制氨苄青霉素或磷霉素诱导的金黄色葡萄球菌L型生长。对马血清进行分级分离后发现，通过漂浮超速离心、凝胶过滤和等电聚焦，抑制活性与高密度脂蛋白共同纯化。还表明这种抑制是可逆的：氨苄青霉素诱导的L型细胞在接种前与脂蛋白组分或血清混合时会失去形成L型菌落的能力，但通过用蛋白酶处理混合物，菌落形成能力可恢复到原始水平，而单纯稀释则无效。生长抑制的机制仍有待阐明。
